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S100A4 Antibody (S100A4/1482)

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Western Blot: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1482).
Western Blot: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1482).
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse ...read more
Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1482).
Flow Cytometry: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Flow Cytometric Analysis of A549 cells using S100A4 Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Western Blot of HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1482).
Flow Cytometry: S100A4 Antibody (S100A4/1482) [NBP2-53270] - Flow Cytometric Analysis of T98G cells using S100A4 Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
S100A4/1482
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54400AF350
Alexa Fluor 405
NBP2-54400AF405
Alexa Fluor 488
NBP2-54400AF488
Alexa Fluor 532
NBP2-54400AF532
Alexa Fluor 594
NBP2-54400AF594
Alexa Fluor 647
NBP2-54400AF647
Alexa Fluor 700
NBP2-54400AF700
Alexa Fluor 750
NBP2-54400AF750
Allophycocyanin
NBP2-54400APC
Allophycocyanin/Cy7
NBP2-54400APCCY7
Biotin
NBP2-54400B
CoraFluor 1
NBP2-54400CL1
DyLight 350
NBP2-54400UV
DyLight 405
NBP2-54400V
DyLight 488
NBP2-54400G
DyLight 550
NBP2-54400R
DyLight 594
NBP2-54400DL594
DyLight 650
NBP2-54400C
DyLight 680
NBP2-54400FR
DyLight 755
NBP2-54400IR
FITC
NBP2-54400F
HRP
NBP2-54400H
Janelia Fluor 525
NBP2-54400JF525
Janelia Fluor 549
NBP2-54400JF549
Janelia Fluor 585
NBP2-54400JF585
Janelia Fluor 635
NBP2-54400JF635
Janelia Fluor 646
NBP2-54400JF646
Janelia Fluor 669
NBP2-54400JF669
PE
NBP2-54400PE
PE/Atto594
NBP2-54400PEATT594
PE/Cy5.5
NBP2-54400PECY55
PE/Cy7
NBP2-54400PECY7
PerCP
NBP2-54400PCP
mFluor Violet 450 SE
NBP2-54400MFV450
mFluor Violet 500 SE
NBP2-54400MFV500
mFluor Violet 610 SE
NBP2-54400MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54400-100ug
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S100A4 Antibody (S100A4/1482) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54400)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment of human S100A4 protein (aa1-200) (exact sequence is proprietary) (Uniprot: P26447)
Localization
Cytoplasmic and Nuclear
Marker
Marker of Tumor Metastasis
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for S100A4 Antibody (S100A4/1482)

  • 18A2
  • 42A
  • Calvasculin
  • CAPL
  • CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
  • fibroblast-specific protein-1,42A
  • FSP1
  • leukemia multidrug resistance associated protein
  • malignant transformation suppression 1
  • Metastasin
  • MTS1
  • murine placental homolog)
  • P9KA
  • PEL98
  • Placental calcium-binding protein
  • Protein Mts1
  • protein S100-A4
  • S100 calcium binding protein A4
  • S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
  • S100 calcium-binding protein A4
  • S100A4

Background

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol S100A4
Uniprot