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RTKN Antibody

Images

 
Western Blot: RTKN Antibody [NBP2-20239] - RTKN antibody detects RTKN protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human RTKN-transfected 293T cells. ...read more
Immunocytochemistry/ Immunofluorescence: RTKN Antibody [NBP2-20239] - RTKN antibody detects RTKN protein at cytoplasm by immunofluorescent analysis.Sample: RTKN-transfected (right) or untransfected (left) HeLa cells ...read more
Western Blot: RTKN Antibody [NBP2-20239] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: RTKN Antibody [NBP2-20239] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: RTKN Antibody [NBP2-20239] - RTKN antibody detects RTKN protein by western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug NCI-H929 whole cell ...read more
Immunocytochemistry/ Immunofluorescence: RTKN Antibody [NBP2-20239] - Analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RTKN Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RTKN. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTKN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for RTKN Antibody

  • B5
  • rhotekin
  • RTKN1

Background

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RTKN
Uniprot