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ROR1 Antibody [Unconjugated]

 (20 Publications)
 (2 Reviews)
 
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Western blot shows lysate of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysate of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) ...read more
MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (AB-108-C, open histogram), ...read more
Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 ...read more
Genetic Strategies: Knockdown of ROR1 in serous ovarian cancer cells decreases migrationA. ROR1 is decreased at the mRNA level following ROR1 siRNA (A) induced knockdown in serous ovarian cancer (OVCAR3) cells. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
FAB2000G-100UG
Biotin
BAF2000
Phycoerythrin
FAB2000P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2000G-100UG
Carrier Free
FAB2000P
Carrier Free
AF2000-SP
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ROR1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR1
Ala24-Glu403
Accession # Q01973
Specificity
Detects human ROR1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2000 in the following applications:
Publications
Read Publications using
AF2000 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR1 Antibody [Unconjugated]

  • EC 2.7.10.1
  • MGC99659
  • neurotrophic tyrosine kinase receptor-related 1
  • Neurotrophic tyrosine kinase, receptor-related 1
  • NTRKR1
  • NTRKR1dJ537F10.1
  • receptor tyrosine kinase-like orphan receptor 1
  • ROR1
  • tyrosine-protein kinase transmembrane receptor ROR1

Background

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ROR1 Antibody
[Unconjugated]
AF2000
ROR1 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready

Publications for ROR1 Antibody (AF2000)(20)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(2)
IHC
(1)
Immunoprecipitation
(6)
Western Blot
(11)
All Applications
Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF2000 Applications Species
Nishita, M;Kamizaki, K;Hoshi, K;Aruga, K;Nishikaku, I;Shibuya, H;Matsumoto, K;Minami, Y; Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression The Journal of biological chemistry 2023-09-11 [PMID: 37703992] (Immunoprecipitation, Human) Immunoprecipitation Human
D Liu, GF Kaufmann, JB Breitmeyer, KA Dickson, DJ Marsh, CE Ford The Anti-ROR1 Monoclonal Antibody Zilovertamab Inhibits the Proliferation of Ovarian and Endometrial Cancer Cells Pharmaceutics, 2022-04-11;14(4):. 2022-04-11 [PMID: 35456672] (Western Blot, Human) Western Blot Human
Y Liu, H Deng, L Liang, G Zhang, J Xia, K Ding, N Tang, K Wang Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway Genes & diseases, 2020-07-25;8(2):232-240. 2020-07-25 [PMID: 33997170] (ICC, IHC, Western Blot, Human) ICC, IHC, Western Blot Human
D Liu, K Gunther, LA Enriquez, B Daniels, TA O'Mara, K Tang, AB Spurdle, CE Ford ROR1 is upregulated in endometrial cancer and represents a novel therapeutic target Sci Rep, 2020-08-17;10(1):13906. 2020-08-17 [PMID: 32807831] (Western Blot, Human) Western Blot Human
Anne Ljungars, Carolin Svensson, Anders Carlsson, Elin Birgersson, Ulla-Carin Tornberg, Björn Frendéus et al. Deep Mining of Complex Antibody Phage Pools Generated by Cell Panning Enables Discovery of Rare Antibodies Binding New Targets and Epitopes Frontiers in Pharmacology 2019-07-30 [PMID: 31417405]
SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1 EBioMedicine, 2019-05-10;0(0):. 2019-05-10 [PMID: 31085100] (ICC, Western Blot, Human) ICC, Western Blot Human
T Yamaguchi, M Hayashi, L Ida, M Yamamoto, C Lu, T Kajino, J Cheng, M Nakatochi, H Isomura, M Yamazaki, M Suzuki, T Fujimoto, T Takahashi ROR1-CAVIN3 interaction required for caveolae-dependent endocytosis and pro-survival signaling in lung adenocarcinoma Oncogene, 2019-03-20;0(0):. 2019-03-20 [PMID: 30894682] (Immunoprecipitation, Human) Immunoprecipitation Human
AH Daneshmane, M Hojjat-Far, A Ghaderi, A Moshfegh, L Hansson, J Schultz, J Vågberg, S Byström, E Olsson, T Olin, A Österborg, H Mellstedt A receptor tyrosine kinase ROR1 inhibitor (KAN0439834) induced significant apoptosis of pancreatic cells which was enhanced by erlotinib and ibrutinib PLoS ONE, 2018-06-01;13(6):e0198038. 2018-06-01 [PMID: 29856777] (Western Blot, Human) Western Blot Human
M Chow, L Gao, JD MacManiman, VT Bicocca, BH Chang, JJ Alumkal, JW Tyner Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL Oncogene, 2018-05-30;0(0):. 2018-05-30 [PMID: 29849118] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
CE Henry, E Llamosas, B Daniels, A Coopes, K Tang, CE Ford ROR1 and ROR2 play distinct and opposing roles in endometrial cancer Gynecol. Oncol., 2018-02-01;0(0):. 2018-02-01 [PMID: 29395309] (Western Blot, Human) Western Blot Human
Show All 20 Publications.

Reviews for ROR1 Antibody (AF2000) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2000:
Filter by Applications
WB
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23593420
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/06/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20597086
SpeciesHuman

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Recent Reviews

4.5
5
1
4
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0
2
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1
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 Verified Customer
01/06/2015
Application: WB
Species: Human
read this review
 
 Verified Customer
01/06/2015
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol ROR1
Uniprot