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Frizzled-6 Antibody

 (18 Publications)
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FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with anti-acetylated-alpha -tubulin antibody (rose). (A1, A2) In the wild-type mice (at P25), FZD6 protein localized asymmetrically to the cell cortex at the level of the apical junctions (arrow in A2). (B1, B2) However, in the mutant mice (at P25), FZD6 protein lost its polarized distribution and was located on the entire membrane at the apical level (arrows in B2).(A3, B3) Schematic diagram of FZD6 protein (red line) distribution in wild-type and Spag6−/− mice. The color codes are the same as that in panel A1 and B1. Bars: A, 5 µm; B, 7.5 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25393619), licensed under a CC-BY license. Not internally tested by R&D Systems.
FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with ...read more
FZD6 protein polarity is lost in mutant tympanic ciliary epithelium.(A–B) The distribution of the core PCP protein, FZD6, was examined by immunofluorescence staining. Ciliated epithelial cells were marked with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1526
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1526-SP
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Frizzled-6 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-6
His19-Val153
Accession # Q61089
Specificity
Detects mouse Frizzled-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Frizzled-6 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8, and rmFrizzled-9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1526 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-6 Antibody

  • frizzled (Drosophila) homolog 6
  • frizzled homolog 6 (Drosophila)
  • Frizzled6
  • Frizzled-6
  • FZ6
  • fz-6
  • FZD6
  • Hfz6
  • seven transmembrane helix receptor

Background

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-6 Antibody (AF1526)(18)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, IHC-P.


Filter By Application
IHC
(8)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Mouse
(10)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF1526 Applications Species
Cetera, M;Sharan, R;Hayward-Lara, G;Phillips, B;Biswas, A;Halley, M;Beall, E;vonHoldt, B;Devenport, D; Region-specific reversal of epidermal planar polarity in the fancy rosette mouse bioRxiv : the preprint server for biology 2023-07-28 [PMID: 37546950] (IHC, Mouse) IHC Mouse
Y Ozono, A Tamura, S Nakayama, E Herawati, Y Hanada, K Ohata, M Takagishi, M Takahashi, T Imai, Y Ohta, K Oshima, T Sato, H Inohara, S Tsukita Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules Scientific Reports, 2021-10-12;11(1):20224. 2021-10-12 [PMID: 34642354] (IHC, Mouse) IHC Mouse
KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia Nature Communications, 2021-05-17;12(1):2861. 2021-05-17 [PMID: 34001891] (IHC, Mouse) IHC Mouse
Andre Landin Malt, Shaylyn Clancy, Diane Hwang, Alice Liu, Connor Smith, Margaret Smith et al. Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3 beta Inhibition Frontiers in Cell and Developmental Biology 4/16/2021 [PMID: 33937247]
Shogo Nakayama, Tomoki Yano, Toshinori Namba, Satoshi Konishi, Maki Takagishi, Elisa Herawati et al. Planar cell polarity induces local microtubule bundling for coordinated ciliary beating Journal of Cell Biology 2021-07-05 [PMID: 33929515]
Sara N Stahley, Lena P Basta, Rishabh Sharan, Danelle Devenport Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes eLife 2021-02-02 [PMID: 33529151]
Kyeong-Hye Moon, Ji-Hyun Ma, Hyehyun Min, Heiyeun Koo, HongKyung Kim, Hyuk Wan Ko et al. Dysregulation of sonic hedgehog signaling causes hearing loss in ciliopathy mouse models eLife 2020-12-31 [PMID: 33382037]
Andre Landin Malt, Arielle K. Hogan, Connor D. Smith, Maxwell S. Madani, Xiaowei Lu Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling Journal of Cell Biology 10/5/2020 [PMID: 32805026]
Satish R. Ghimire, Michael R. Deans Frizzled3 and Frizzled6 Cooperate with Vangl2 to Direct Cochlear Innervation by Type II Spiral Ganglion Neurons The Journal of Neuroscience 2019-10-09 [PMID: 31462532]
Koshi Kunimoto, Roy D. Bayly, Eszter K. Vladar, Tyson Vonderfecht, Anna-Rachel Gallagher, Jeffrey D. Axelrod Disruption of Core Planar Cell Polarity Signaling Regulates Renal Tubule Morphogenesis but Is Not Cystogenic Current Biology 2017-10-23 [PMID: 29033332]
Show All 18 Publications.

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Bioinformatics

Gene Symbol FZD6
Uniprot