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ROCK2 Antibody

Images

 
Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ROCK2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROCK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 26330114). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ROCK2 Protein (NBP1-86124PEP)
Publications
Read Publications using
NBP1-86124 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23319410)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ROCK2 Antibody

  • coiled-coil-containing protein kinase 2
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA0619
  • p164 ROCK-2
  • Rho kinase 2
  • rho-associated protein kinase 2
  • Rho-associated, coiled-coil containing protein kinase 2
  • ROCK2
  • ROCK-II
  • ROKalpha

Background

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for ROCK2 Antibody (NBP1-86124)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for ROCK2 Antibody (NBP1-86124)

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Blogs on ROCK2.

TGF-beta for treating degenerative intervertebral disc disease
By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain...  Read full blog post.

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Bioinformatics

Gene Symbol ROCK2