![Western Blot: RIPX Antibody [NBP1-89614] - Analysis using Anti-RUFY3 antibody NBP1-89614(A) shows similar pattern to independent antibody NBP2-48558 (B).](http://images.novusbio.com/fullsize/RIPX-Antibody-Western-Blot-NBP1-89614-img0009.jpg "Western Blot: RIPX Antibody [NBP1-89614] - Analysis using Anti-RUFY3 antibody NBP1-89614(A) shows similar pattern to independent antibody NBP2-48558 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: MLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQRLRQAERSRQSAELDNRLFKQDFGDKINSLQLEVEELTRQRNQLEL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RUFY3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publications using NBP1-89614 | Applications | Species |
|---|---|---|
| Keren-Kaplan T, Saric A, Ghosh S et al. RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin Nature communications 2022-03-21 [PMID: 35314674] | ||
| Char R, Liu Z, Davieau M et al. Rufy3 regulates anti-bacterial responses by controlling endolysosomes perinuclear positioning in activated phagocytes. Research Square 2022-10-05 (ICC/IF, Human, Mouse) Details: Dilution used in ICC/IF 1:1000 |
ICC/IF | Human, Mouse |
| Sasazawa Y, Souma S, Furuya N et al. Oxidative stress-induced phosphorylation of JIP4 regulates lysosomal positioning in coordination with TRPML1 and ALG2 The EMBO Journal 2022-10-11 [PMID: 36394115] (WB, Human) | WB | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Human and Rat | 08/21/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RUFY3 |
![Western Blot: RIPX Antibody [NBP1-89614] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/RIPX-Antibody-Western-Blot-NBP1-89614-img0011.jpg "Western Blot: RIPX Antibody [NBP1-89614] - Analysis in human cell line SCLC-21H.")
![Immunocytochemistry/Immunofluorescence: RIPX Antibody [NBP1-89614] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RIPX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89614-img0004.jpg "Immunocytochemistry/Immunofluorescence: RIPX Antibody [NBP1-89614] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RIPX Antibody [NBP1-89614] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/RIPX-Antibody-Immunohistochemistry-Paraffin-NBP1-89614-img0007.jpg "Immunohistochemistry-Paraffin: RIPX Antibody [NBP1-89614] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.")
![Western Blot: RIPX Antibody [NBP1-89614] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/RIPX-Antibody-Western-Blot-NBP1-89614-img0006.jpg "Western Blot: RIPX Antibody [NBP1-89614] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Western Blot: RIPX Antibody [NBP1-89614] - HEK 293 cells non-transfected (lane 1) and transfected with rat Rufy3 full length plasmid (lane 2). Band at 70kDa isoform 3 of Rufy3. Band at 55kDa isoform 1 of Rufy3. Image from a verified customer review.](http://images.novusbio.com/fullsize/RIPX-Antibody-Western-Blot-NBP1-89614-img0010.jpg "Western Blot: RIPX Antibody [NBP1-89614] - HEK 293 cells non-transfected (lane 1) and transfected with rat Rufy3 full length plasmid (lane 2). Band at 70kDa isoform 3 of Rufy3. Band at 55kDa isoform 1 of Rufy3. Image from a verified customer review.")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Simple_Western_21000.jpg)
![Western Blot STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Western_Blot_5993.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Knockout_Validated_21531.jpg)
![Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]](https://images.novusbio.com/images2/TFF2_MAB4077_Immunohistochemistry_21485.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
