![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - Parkin knockout improves the decline of mitochondrial dynamics proteins in SOD1-G93A spinal cord, but mitochondrial protein ubiquitination profiles and March5 and Mul1 levels are unaffected A, B Western blots (A) and quantification (B) of Miro1 in spinal cord homogenates at disease end stage. Beta-actin is used as loading reference. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0005.jpg "Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - Parkin knockout improves the decline of mitochondrial dynamics proteins in SOD1-G93A spinal cord, but mitochondrial protein ubiquitination profiles and March5 and Mul1 levels are unaffected A, B Western blots (A) and quantification (B) of Miro1 in spinal cord homogenates at disease end stage. Beta-actin is used as loading reference. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4H4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | RHOT1 (NP_060777, 483 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP |
| Specificity | RHOT1 - ras homolog gene family, member T1 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RHOT1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0004.jpg "Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.")
![ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rhot1-Antibody-4H4-ELISA-H00055288-M01-img0003.jpg "ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")