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RGS3 Antibody

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Immunocytochemistry/ Immunofluorescence: RGS3 Antibody [NBP3-17760] - Staining of human cell line U-2 OS shows localization to plasma membrane.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RGS3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DTIPEESGSPSKGKSYTGLGKKSRPMKTVQTMKGHGNYQNCPVVRPHATHSSYGTYVTLAPKVLVFPVFVQPLDLCNPART
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RGS3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RGS3 Antibody

  • C2PA
  • FLJ20370
  • FLJ31516
  • FLJ90496
  • PDZ-RGS3
  • regulator of G-protein signaling 3
  • regulator of G-protein signalling 3
  • RGP3

Background

RGS 3 (Regulator of G protein Signaling 3) is a 519 residue protein that is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for RGS3 Antibody (NBP3-17760) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RGS3