![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Knockdown-Validated-H00005997-M01-img0011.jpg "Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 4C4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RGS2 (AAH07049, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT |
| Specificity | RGS2 - regulator of G-protein signalling 2, 24kDa |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RGS2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RGS2 Antibody (H00005997-M01)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0012.jpg "Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Immunohistochemistry-Paraffin-H00005997-M01-img0005.jpg "Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.")
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0007.jpg "Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.")
![ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-ELISA-H00005997-M01-img0008.jpg "ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.")
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415394536.jpg "Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415482256.jpg "Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Flow Cytometry Lgr6 Antibody (918719) [Unconjugated]](https://images.novusbio.com/images2/Lgr6_MAB8458_Flow_Cytometry_16097.jpg)
![Immunocytochemistry Lgr6 Antibody (918719) [Unconjugated]](https://images.novusbio.com/images2/Lgr6_MAB8458_Immunocytochemistry_16415.jpg)
