![Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Inverse Arg2-RGS16 regulatory relationship is identified by unbiased transcriptomics. RGS16 gene expression and protein abundance in HFrD-fed (f) AAV8-control and AAV8-Arg2 mouse livers. For box plots, the midline represents the median, boxes represent the interquartile range and whiskers show the full range of values. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student's t-test Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09642-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Western-Blot-NBP2-01584-img0009.jpg "Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Inverse Arg2-RGS16 regulatory relationship is identified by unbiased transcriptomics. RGS16 gene expression and protein abundance in HFrD-fed (f) AAV8-control and AAV8-Arg2 mouse livers. For box plots, the midline represents the median, boxes represent the interquartile range and whiskers show the full range of values. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student's t-test Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09642-8), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human RGS16(NP_002919) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RGS16 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 22.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-01584 | Applications | Species |
|---|---|---|
| Zhang, Y;Higgins, CB;Fortune, HM;Chen, P;Stothard, AI;Mayer, AL;Swarts, BM;DeBosch, BJ; Hepatic arginase 2 (Arg2) is sufficient to convey the therapeutic metabolic effects of fasting Nat Commun 2019-04-08 [PMID: 30962478] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for RGS16 Antibody (NBP2-01584)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Immunocytochemistry-Immunofluorescence-NBP2-01584-img0007.jpg "Immunocytochemistry/Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16.")
![Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Immunohistochemistry-Paraffin-NBP2-01584-img0006.jpg "Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.")
![Flow Cytometry: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of Jurkat cells, using anti-RGS16 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Flow-Cytometry-NBP2-01584-img0001.jpg "Flow Cytometry: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of Jurkat cells, using anti-RGS16 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS16.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Western-Blot-NBP2-01584-img0002.jpg "Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS16.")
![Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RGS16 monoclonal antibody.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Western-Blot-NBP2-01584-img0008.jpg "Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RGS16 monoclonal antibody.")
![Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human breast tissue using anti-RGS16 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Immunohistochemistry-Paraffin-NBP2-01584-img0003.jpg "Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human breast tissue using anti-RGS16 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human endometrium tissue using anti-RGS16 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Immunohistochemistry-Paraffin-NBP2-01584-img0004.jpg "Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human endometrium tissue using anti-RGS16 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human Kidney tissue using anti-RGS16 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGS16-Antibody-OTI4E5-Immunohistochemistry-Paraffin-NBP2-01584-img0005.jpg "Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human Kidney tissue using anti-RGS16 mouse monoclonal antibody.")
![Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Hepatic RGS16 reconstitution reverses Arg2-mediated improvements in peripheral fat accumulation & glucose homeostasis. a Experimental schematic used to test the role of AAV8-mediated mouse Arg2 & Ad-RGS16 overexpression in db/db mice. b Body weight & c body composition of AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). d VO2, VCO2, energy expenditure, & RER during light & dark cycles in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). e Serum insulin (left), glucose (middle), & HOMA2 IR (right) in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). f Intraperitoneal GTT & ITT (n = 5 mice per group). g Western blot analysis of total & phosphorylated AKT in liver samples from db/db AAV8-Control & AAV8-Arg2 mice (n = 4 mice per group). For box plots, the midline represents the median, boxes represent the interquartile range & whiskers show the full range of values. For bar graphs, data represent mean+s.e.m. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student’s t-test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30962478), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-01584_mouse-rgs16-mab-oti4e5-31020241613614.jpg "Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Hepatic RGS16 reconstitution reverses Arg2-mediated improvements in peripheral fat accumulation & glucose homeostasis. a Experimental schematic used to test the role of AAV8-mediated mouse Arg2 & Ad-RGS16 overexpression in db/db mice. b Body weight & c body composition of AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). d VO2, VCO2, energy expenditure, & RER during light & dark cycles in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). e Serum insulin (left), glucose (middle), & HOMA2 IR (right) in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). f Intraperitoneal GTT & ITT (n = 5 mice per group). g Western blot analysis of total & phosphorylated AKT in liver samples from db/db AAV8-Control & AAV8-Arg2 mice (n = 4 mice per group). For box plots, the midline represents the median, boxes represent the interquartile range & whiskers show the full range of values. For bar graphs, data represent mean+s.e.m. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student’s t-test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30962478), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")