Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily (1, 2). Mature rat CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 217 aa extracellular region. The extracellular domain of rat CD40 Ligand shares 75% and 93% amino acid (aa) sequence identity with the human and mouse proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (3). The 18 kDa soluble form (aa 112-260)  arises from proteolytic processing and retains the ability to bind and activate CD40 (4, 5). Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes (2). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).

Array 8414-CL

• CD40 Ligand/TNFSF5 Antibodies
• CD40 Ligand/TNFSF5 ELISA Kits
• CD40 Ligand/TNFSF5 Lysate
• CD40 Ligand/TNFSF5 Proteins
• CD40 Ligand/TNFSF5 Support Product
• CD40 Ligand/TNFSF5 ELISAs
• CD40 Ligand/TNFSF5 Proteins and Enzymes