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Recombinant Rat CD34 Fc Chimera Protein, CF

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Summary
Reactivity RtSpecies Glossary
Format
Carrier-Free

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Recombinant Rat CD34 Fc Chimera Protein, CF Summary

Details of Functionality
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived rat CD34 protein
Rat CD34
(Glu37-Leu292)
Accession # NP_001100672		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Glu37
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Innovator Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity not tested
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
98-111 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CD34 Fc Chimera Protein, CF

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 is a glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Rat CD33 cDNA encodes a 386 amino acid (aa) precursor that contains a 36 aa signal sequence, a 256 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, rat CD34 shares 81% and 52% aa sequence identity with mouse and human CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
  1. Civin C.I. et al. (1984) J. Immunol. 133:157.
  2. Katz F. et al. (1985) Leuk. Res. 9:191.
  3. Andrews R.G. et al. (1986) Blood 67:842.
  4. Young P.E. et al. (1995) Blood 85:96.
  5. Krause D.S. et al. (1996) Blood 87:1.
  6. Sidney L.E. et al. (2014) Stem Cells 32:1380.

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FAQs for CD34 (9654-CD). (Showing 1 - 1 of 1 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

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