Recombinant Porcine IL-6 Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 58%, 39%, and 39% aa sequence identity with human, mouse, and rat IL-6, respectively IL-6 (6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 9). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 10).

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), ELISA Standard, In Vivo.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using 686-PI	Applications	Species
SY Yin, BM Sun, T Xu, X Liu, LJ Huo, X Zhang, J Zhou, YL Miao CHIR99021 and rpIL6 promote porcine parthenogenetic embryo development and blastocyst quality Theriogenology, 2020-08-17;158(0):470-476. 2020-08-17 [PMID: 33049572] (Bioassay, Porcine)	Bioassay	Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Standard, Porcine)	ELISA Standard	Porcine
T Huang, X Huang, W Chen, J Yin, B Shi, F Wang, W Feng, M Yao MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-gamma in regulation of fecal bacteria shedding counts in pig BMC Vet. Res., 2019-06-11;15(1):195. 2019-06-11 [PMID: 31186019] (In Vivo, Porcine)	In Vivo	Porcine
B Ulmasov, BA Neuschwand, J Lai, V Monastyrsk, T Bhat, MP Yates, J Oliva, MJ Prinsen, PG Ruminski, DW Griggs Inhibitors of Arg-Gly-Asp-Binding Integrins Reduce Development of Pancreatic Fibrosis in Mice Cell Mol Gastroenterol Hepatol, 2016-03-16;2(4):499-518. 2016-03-16 [PMID: 28174730] (Bioassay, Porcine)	Bioassay	Porcine
S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA (Standard), Porcine)	ELISA (Standard)	Porcine
Interferon regulatory factor 3 is a key regulation factor for inducing the expression of SAMHD1 in antiviral innate immunity Sci Rep, 2016-07-14;6(0):29665. 2016-07-14 [PMID: 27411355] (Bioassay, Porcine)	Bioassay	Porcine
Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin. J. Interferon Cytokine Res., 2007-09-01;27(9):757-66. 2007-09-01 [PMID: 17892397] (Bioassay, Porcine)	Bioassay	Porcine
Saurer L, McCullough KC, Summerfield A In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid. J. Immunol., 2007-09-15;179(6):3504-14. 2007-09-15 [PMID: 17785784] (Bioassay, Porcine)	Bioassay	Porcine
Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2634-43. 2007-06-01 [PMID: 17525194] (Bioassay, Porcine)	Bioassay	Porcine
Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. J. Virol., 2006-01-01;80(1):372-82. 2006-01-01 [PMID: 16352562] (ELISA (Standard))	ELISA (Standard)
Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004-04-01;100(4):861-70. 2004-04-01 [PMID: 15087621] (ELISA (Standard))	ELISA (Standard)
Show All 11 Publications. Collapse Publications.

Array 686-PI

• IL-6 Antibodies
• IL-6 Antibody Pair
• IL-6 ELISA Kits
• IL-6 Lysate
• IL-6 Proteins
• IL-6 Proteins and Enzymes
• IL-6 ELISAs