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Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

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When Recombinant Mouse PD‑L2/B7‑DC (K113S) FcChimera (Catalog # 10021-PL) is immobilized at 2 µg/mL,Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
When Recombinant Mouse PD‑L2/B7‑DC (K113S) FcChimera (Catalog # 10021-PL) is immobilized at 2 µg/mL,Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse RGM‑B (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera (Catalog #10021-PL) weakly binds to Recombinant Mouse PD-1.
Source
Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
Mouse PD-L2
(Leu20-Arg219) (Lys113Ser)
Accession # Q9WUL5		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Leu20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-84 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

  • B7-DC
  • bA574F11.2
  • Btdc
  • Butyrophilin B7-DC
  • Butyrophilin-like Protein
  • CD273 antigen
  • CD273
  • CD273PD-1 ligand 2
  • MGC142240
  • PD-1-ligand 2
  • PDCD1L2MGC142238
  • PDCD1LG2
  • PDL2
  • PD-L2
  • PDL2B7DC
  • PD-L2PDCD1 ligand 2
  • programmed cell death 1 ligand 2
  • Programmed death ligand 2

Background

Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse PD-L2 shares 72% and 95% aa sequence identity with human and rat PD‑L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3‑5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to repulsive guidance molecule family member b (RGMb) on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). Replacement of lysine residue at position 113 with serine (K113S) has been reported to result in a loss of binding capacity to PD-1, while retaining its T-cell stimulatory function through its interaction with RGMb (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
  14. Nie, X. et al. (2017) Cell. Mol. Immunol. 14:1.

Show all background references

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