Novus Biologicals products are now on bio-techne.com

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Images

 
When Recombinant Mouse PD‑L2/B7‑DC (K113S) FcChimera (Catalog # 10021-PL) is immobilized at 2 µg/mL,Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
2 μg/lane of Recombinant Mouse PD‑L2/B7‑DC (K113S) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 64-84 kDa and ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse RGM‑B (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera (Catalog #10021-PL) weakly binds to Recombinant Mouse PD-1.
Source
Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
Mouse PD-L2
(Leu20-Arg219) (Lys113Ser)
Accession # Q9WUL5
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Leu20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-84 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

  • B7-DC
  • bA574F11.2
  • Btdc
  • Butyrophilin B7-DC
  • Butyrophilin-like Protein
  • CD273 antigen
  • CD273
  • CD273PD-1 ligand 2
  • MGC142240
  • PD-1-ligand 2
  • PDCD1L2MGC142238
  • PDCD1LG2
  • PDL2
  • PD-L2
  • PDL2B7DC
  • PD-L2PDCD1 ligand 2
  • programmed cell death 1 ligand 2
  • Programmed death ligand 2

Background

Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse PD-L2 shares 72% and 95% aa sequence identity with human and rat PD‑L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3‑5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to repulsive guidance molecule family member b (RGMb) on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). Replacement of lysine residue at position 113 with serine (K113S) has been reported to result in a loss of binding capacity to PD-1, while retaining its T-cell stimulatory function through its interaction with RGMb (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
  14. Nie, X. et al. (2017) Cell. Mol. Immunol. 14:1.

Customers Who Viewed This Item Also Viewed...

NBP1-76769
Species: Hu, Mu, Rt
Applications: Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, KD, WB
AF1086
Species: Hu
Applications: Block, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, WB
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
MAB7158
Species: Hu, Mu
Applications: IHC, WB
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
202-IL
Species: Hu
Applications: BA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
DY417
Species: Mu
Applications: ELISA
MAB342
Species: Hu
Applications: AgAct, ICC, WB
AF165
Species: Hu
Applications: CyTOF-ready, Flow, WB
6507-IL/CF
Species: Hu
Applications: BA
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
AF169
Species: Hu
Applications: IHC, WB

Publications for PD-L2/B7-DC/PDCD1LG2 (10021-PL) (0)

There are no publications for PD-L2/B7-DC/PDCD1LG2 (10021-PL).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PD-L2/B7-DC/PDCD1LG2 (10021-PL) (0)

There are no reviews for PD-L2/B7-DC/PDCD1LG2 (10021-PL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for PD-L2/B7-DC/PDCD1LG2 (10021-PL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PD-L2/B7-DC/PDCD1LG2 Products

Blogs on PD-L2/B7-DC/PDCD1LG2

There are no specific blogs for PD-L2/B7-DC/PDCD1LG2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot