Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Bioassay, ELISA (Capture), ELISA Capture, ELISA Standard, Enzyme Assay, Flow Cytometry, In Vivo, Surface Plasmon Resonance.

Showing Publications 1 - 10 of 23. Show All 23 Publications. Collapse Publications.

Publications using 1019-B7	Applications	Species
Zhong, W;Lu, Y;Han, X;Yang, J;Qin, Z;Zhang, W;Yu, Z;Wu, B;Liu, S;Xu, W;Zheng, C;Schuchter, LM;Karakousis, GC;Mitchell, TC;Amaravadi, R;Flowers, AJ;Gimotty, PA;Xiao, M;Mills, G;Herlyn, M;Dong, H;Mitchell, MJ;Kim, J;Xu, X;Guo, W; Upregulation of exosome secretion from tumor-associated macrophages plays a key role in the suppression of anti-tumor immunity Cell reports 2023-10-07 [PMID: 37805922] (ELISA Standard, Human)	ELISA Standard	Human
Corria-Osorio J, Carmona SJ, Stefanidis E, Andreatta M, Ortiz-Miranda Y, Muller T, Rota IA, Crespo I, Seijo B, Castro W, Jimenez-Luna C, Scarpellino L, Ronet C, Spill A, Lanitis E, Romero P, Luther SA, Irving M, Coukos G Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8+ T cells Nat Immunol. 2023; 24(5): 869–883. 2023-04-20 [PMID: 37081150] (Bioassay, Mouse)	Bioassay	Mouse
KA Meerschaer, BS Edwards, AY Epouhe, B Jefferson, R Friedman, OL Babyok, JK Moy, F Kehinde, C Liu, CJ Workman, DAA Vignali, KM Albers, HR Koerber, MS Gold, BM Davis, NN Scheff, JL Saloman Neuronally expressed PDL1, not PD1, suppresses acute nociception Brain, Behavior, and Immunity, 2022-09-08;106(0):233-246. 2022-09-08 [PMID: 36089217] (In Vivo, Mouse)	In Vivo	Mouse
C Martins, M Silva, E Rasbach, P Singh, Y Itoh, JB Williams, E Statham, A Meurer, DV Martinez, A Brandenbur, MV Heppt, SR Barthel, T Schatton Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells Scientific Reports, 2022-07-21;12(1):12491. 2022-07-21 [PMID: 35864188] (Bioassay, Mouse)	Bioassay	Mouse
CM Bryan, GJ Rocklin, MJ Bick, A Ford, S Majri-Morr, AV Kroll, CJ Miller, L Carter, I Goreshnik, A Kang, F DiMaio, KV Tarbell, D Baker Computational design of a synthetic PD-1 agonist Proceedings of the National Academy of Sciences of the United States of America, 2021-07-20;118(29):. 2021-07-20 [PMID: 34272285] (Bioassay, Mouse)	Bioassay	Mouse
A Thanabalas, AJ Chiang, C Morehouse, M Camara, S Hawkins, AE Keller, AC Koksal, CS Caceres, AA Berlin, N Holoweckyj, VN Takahashi, L Cheng, M de Los Rey, M Pelletier, AC Patera, B Sellman, S Hess, M Marelli, CC Boo, TS Cohen, A DiGiandome PD-L1+ neutrophils contribute to injury-induced infection susceptibility Science Advances, 2021-03-05;7(10):. 2021-03-05 [PMID: 33674305] (Bioassay, Mouse)	Bioassay	Mouse
A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models PLoS ONE, 2020-09-24;15(9):e0239595. 2020-09-24 [PMID: 32970735] (Bioassay, Human)	Bioassay	Human
S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs Pharmaceutics, 2020-06-26;12(6):. 2020-06-26 [PMID: 32604843] (ELISA Capture, Mouse)	ELISA Capture	Mouse
SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex PLoS ONE, 2020-06-04;15(6):e0233578. 2020-06-04 [PMID: 32497097] (Bioassay, Mouse)	Bioassay	Mouse
LP Garo, AK Ajay, M Fujiwara, V Beynon, C Kuhn, G Gabriely, S Sadhukan, R Raheja, S Rubino, HL Weiner, G Murugaiyan Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity Cell Rep, 2019-09-24;28(13):3353-3366.e5. 2019-09-24 [PMID: 31553906] (In Vivo, Mouse)	In Vivo	Mouse
M Nakajima, Y Sakoda, K Adachi, H Nagano, K Tamada Improved survival of chimeric antigen receptor-engineered T (CAR-T) and tumor-specific T cells caused by anti-programmed cell death protein 1 single-chain variable fragment-producing CAR-T cells Cancer Sci., 2019-09-17;110(10):3079-3088. 2019-09-17 [PMID: 31432594] (ELISA (Capture), Flow Cytometry, Mouse)	ELISA (Capture), Flow Cytometry	Mouse
A von Knethe, A Schäfer, L Kuchler, T Knape, U Christen, E Hintermann, B Fi beta lthaler, K Schröder, RP Brandes, B Genz, K Abshagen, BM Pützer, LK Sha, A Weigert, SN Syed, M Schulz, AM Shah, A Ernst, M Putyrski, F Finkelmeie, M Pesic, F Greten, M Hogardt, VAJ Kempf, S Gunne, MJ Parnham, B Brüne Tolerizing CTL by Sustained Hepatic PD-L1 Expression Provides a New Therapy Approach in Mouse Sepsis Theranostics, 2019-03-16;9(7):2003-2016. 2019-03-16 [PMID: 31037153] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
X Xue, J Wu, J Li, J Xu, H Dai, C Tao, C Li, J Hu Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing Biosci. Rep., 2018-11-23;0(0):. 2018-11-23 [PMID: 30341238] (In Vivo, Mouse)	In Vivo	Mouse
H Tsukamoto, K Fujieda, A Miyashita, S Fukushima, T Ikeda, Y Kubo, S Senju, H Ihn, Y Nishimura, H Oshiumi Combined blockade of IL-6 and PD-1/PD-L1 signaling abrogates mutual regulation of their immunosuppressive effects in the tumor microenvironment Cancer Res., 2018-07-02;0(0):. 2018-07-02 [PMID: 29967259] (Bioassay, Mouse)	Bioassay	Mouse
S Nakagawa, S Serada, R Kakubari, K Hiramatsu, T Sugase, S Matsuzaki, S Matsuzaki, Y Ueda, K Yoshino, T Ohkawara, M Fujimoto, T Kishimoto, T Kimura, T Naka Intratumoral delivery of an adenoviral vector carrying the SOCS-1 gene enhances T cell-mediated anti-tumor immunity by suppressing PD-L1 Mol. Cancer Ther., 2018-06-11;0(0):. 2018-06-11 [PMID: 29891489] (In Vivo, Mouse)	In Vivo	Mouse
J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies Cancer Res, 2017-03-10;0(0):. 2017-03-10 [PMID: 28283653] (In Vivo, Mouse)	In Vivo	Mouse
H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors PLoS Pathog, 2017-01-03;13(1):e1006120. 2017-01-03 [PMID: 28046066]
Jean-Baptiste Marchand Vectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition Oncoimmunology, 2016-09-09;5(10):e1220467. 2016-09-09 [PMID: 27853644] (Bioassay, Mouse)	Bioassay	Mouse
Motoaki Sano Obesity accelerates T cell senescence in murine visceral adipose tissue J. Clin. Invest., 2016-11-07;0(0):. 2016-11-07 [PMID: 27820698] (Bioassay, Mouse)	Bioassay	Mouse
McAlees J, Lajoie S, Dienger K, Sproles A, Richgels P, Yang Y, Khodoun M, Azuma M, Yagita H, Fulkerson P, Wills-Karp M, Lewkowich I Differential control of CD4(+) T-cell subsets by the PD-1/PD-L1 axis in a mouse model of allergic asthma. Eur J Immunol, 2015-02-26;45(4):1019-29. 2015-02-26 [PMID: 25630305] (Bioassay, Mouse)	Bioassay	Mouse
Xiao , Yanping, Yu , Sanhong, Zhu , Baogong, Bedoret , Denis, Bu , Xia, Francisco , Loise M, Hua , Ping, Duke-Cohan , Jonathan, Umetsu , Dale T, Sharpe , Arlene H, DeKruyff , Rosemari, Freeman , Gordon J RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014-04-21;211(5):943-59. 2014-04-21 [PMID: 24752301] (ELISA (Capture), Mouse)	ELISA (Capture)	Mouse
Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli. Protein Expr Purif, 2013-11-08;94(0):60-6. 2013-11-08 [PMID: 24215864] (Bioassay, Enzyme Assay, Mouse)	Bioassay, Enzyme Assay	Mouse
Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 2007-07-12;27(1):111-22. 2007-07-12 [PMID: 17629517] (Surface Plasmon Resonance)	Surface Plasmon Resonance
Show All 23 Publications. Collapse Publications.

Array 1019-B7

• PD-L1 Antibodies
• PD-L1 Antibody Pairs
• PD-L1 Lysate
• PD-L1 Proteins
• PD-L1 Proteins and Enzymes
• PD-L1 ELISAs