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Recombinant Mouse Midkine Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Midkine Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Midkine, immobilized at 125-250 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
E. coli-derived mouse Midkine protein
Lys23-Asp140, with an N-terminal Met
Accession #
N-terminal Sequence
Met 
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15 kDa, reducing conditions
Publications
Read Publications using
9760-MD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Midkine Protein, CF

  • Amphiregulin-associated protein
  • ARAP
  • MDK
  • MEK
  • Midgestation and kidney protein
  • midkine (neurite growth-promoting factor 2)
  • Midkine
  • MK1
  • MKARAP
  • NEGF2
  • NEGF2FLJ27379
  • Neurite outgrowth-promoting factor 2
  • Neurite outgrowth-promoting protein

Background

Midkine (MK), also known as Neurite Growth Promoting Factor 2 (NEGF2), belongs to a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting of MK and pleiotrophin (1). MK is a highly basic, non-glycosylated polypeptide consisting of two domains stabilized by five intrachain disulfide bonds (2). Mature mouse MK is 118 amino acids (aa) in length, approximately 13 kDa, and shares 85% and 95% aa sequence identity with the human and rat protein, respectively. MK was originally identified as being over-expressed during embryogenesis but having minimal expression in adult tissue (3). While early evidence suggested MK promoted neurite outgrowth (4), MK has since been implicated in diverse biological processes ranging from angiogenesis and neurogenesis to inflammation and disease (5, 6). Depending on the function, MK signals through a wide range of varied receptors from Receptor-like Protein Tyrosine Phosphatase beta (RPTP-beta ) to syndecans to Lipoprotein receptor-related proteins (Lrp1) (5, 6). MK may play a significant role in tumorigenesis as over-expression has been observed in many cancers and research has focused on the utility of using MK as a biomarker for cancer and other diseases (5, 7, 8). The presence of MK in the senile plaques of patients with Alzheimer's disease is believed to suppress progression of the disease (9), while MK over-expression in pancreatic or breast cancer is associated with poor prognosis (7, 10, 11).
  1. Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
  2. Fabri L et al. (1993) J Chromatogr. 646:213.
  3. Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.
  4. Asai, T. et al. (1997) Biochem. Biophys. Res. Commun. 236:66.
  5. Muramatsu, T. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86:410.
  6. Sorrelle N et al. (2017) J Leukoc Biol. 102:277.
  7. Wellstein, A. (2012) Front Oncol. 2:192.
  8. Kadomatsu K and Muramatsu T (2004) Cancer Lett. 204:127.
  9. Salama RH et al. (2005) Prog Neuropsychopharmacol Biol Psychiatry. 29:611.
  10. Jono H and Ando Y (2010) Cancers 2:624.
  11. Ibusuki M et al (2009) Cancer Sci. 100:1735.

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