Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Midkine, immobilized at 125-250 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
E. coli-derived mouse Midkine protein Lys23-Asp140, with an N-terminal Met
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15 kDa, reducing conditions
Publications
Read Publications using 9760-MD in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Midkine Protein, CF
Amphiregulin-associated protein
ARAP
MDK
MEK
Midgestation and kidney protein
midkine (neurite growth-promoting factor 2)
Midkine
MK1
MKARAP
NEGF2
NEGF2FLJ27379
Neurite outgrowth-promoting factor 2
Neurite outgrowth-promoting protein
Background
Midkine
(MK), also known as Neurite Growth Promoting Factor 2 (NEGF2), belongs to
a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting
of MK and pleiotrophin (1). MK is a highly basic, non-glycosylated polypeptide consisting
of two domains stabilized by five intrachain disulfide bonds (2). Mature mouse MK
is 118 amino acids (aa) in length, approximately 13 kDa, and shares 85% and 95%
aa sequence identity with the human and rat protein, respectively. MK was originally identified
as being over-expressed during embryogenesis but having minimal expression in
adult tissue (3). While early evidence suggested MK promoted neurite outgrowth
(4), MK has since been implicated in diverse biological processes ranging from angiogenesis and
neurogenesis to inflammation and disease (5, 6). Depending
on the function, MK signals through a wide range of varied receptors from Receptor-like
Protein Tyrosine Phosphatase beta (RPTP-beta ) to syndecans to Lipoprotein
receptor-related proteins (Lrp1) (5, 6).
MK may play a significant role in tumorigenesis as over-expression has been observed in many
cancers and research has focused on the utility of using
MK as a biomarker for cancer
and other diseases (5, 7, 8). The
presence of MK in the senile plaques of patients with Alzheimer's disease is
believed to suppress progression of the disease (9), while MK over-expression
in pancreatic or breast cancer is associated with poor prognosis (7, 10, 11).
Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
Fabri L et al. (1993) J Chromatogr. 646:213.
Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.
Asai, T. et al. (1997) Biochem. Biophys. Res. Commun. 236:66.
Muramatsu, T. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86:410.
Sorrelle N et al. (2017) J Leukoc Biol. 102:277.
Wellstein, A. (2012) Front Oncol. 2:192.
Kadomatsu K and Muramatsu T (2004) Cancer Lett. 204:127.
Salama RH et al. (2005) Prog Neuropsychopharmacol Biol Psychiatry. 29:611.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Recombinant Mouse Midkine Protein, CF and receive a gift card or discount.