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Recombinant Mouse Galectin-3 Protein

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Recombinant Mouse Galectin-3 (Catalog # 1197-GA) agglutinates human red blood cells. The ED50 for this effectis 5‑20µg/mL.
Recombinant Mouse Galectin-3 (Catalog # 1197-GA) agglutinates human red blood cells. The ED50 for this effectis 5‑20µg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
1197-GA-050/CF
Carrier Free
1197-GA-01M/CF
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Recombinant Mouse Galectin-3 Protein Summary

Details of Functionality
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 5‑20 µg/mL.
Source
E. coli-derived mouse Galectin-3 protein
Ala2-Ile264
Accession #
N-terminal Sequence
Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
Lgals3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1197-GA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Galectin-3 Protein

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the cross-linking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).
  1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
  4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
  5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
  6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
  7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
  8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
  9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
  10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
  11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
  12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
  13. Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
  14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
  15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
  16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
  17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
  18. Shin, T. (2013) Acta Histochem. 115:407.

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Publications for Galectin-3 (1197-GA)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Porcine, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(7)
In Vivo
(1)
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Human
(2)
Mouse
(3)
Porcine
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using 1197-GA Applications Species
Sideris-Lampretsas, G;Oggero, S;Zeboudj, L;Silva, R;Bajpai, A;Dharmalingam, G;Collier, DA;Malcangio, M; Galectin-3 activates spinal microglia to induce inflammatory nociception in wild type but not in mice modelling Alzheimer's disease Nature communications 2023-06-22 [PMID: 37349313] (In Vivo, Transgenic Mouse) In Vivo Transgenic Mouse
C Bülck, EEL Nyström, T Koudelka, M Mannbar-Fr, G Andresen, M Radhouani, F Tran, F Scharfenbe, F Schrell, F Armbrust, E Dahlke, B Zhao, A Vervaeke, F Theilig, P Rosenstiel, P Starkl, SP Rosshart, H Fickensche, A Tholey, GC Hansson, C Becker-Pau Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis Science Advances, 2023-03-31;9(13):eadf4055. 2023-03-31 [PMID: 37000885] (Bioassay, Human) Bioassay Human
NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3 Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7. 2021-02-01 [PMID: 33538769] (Bioassay, Porcine) Bioassay Porcine
Jia W, Kidoya H, Yamakawa D, Naito H, Takakura N Galectin-3 accelerates M2 macrophage infiltration and angiogenesis in tumors. Am J Pathol, 2013-03-13;182(5):1821-31. 2013-03-13 [PMID: 23499465] (Bioassay, Mouse) Bioassay Mouse
Mackinnon , Alison C, Liu , Xiaojun, Hadoke , Patrick, Miller , Mark R, Newby , David E, Sethi , Tariq Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice. Glycobiology, 2013-02-19;23(6):654-63. 2013-02-19 [PMID: 23426722] (Bioassay, Mouse) Bioassay Mouse
Li Y, Komai-Koma M, Gilchrist DS, Hsu DK, Liu FT, Springall T, Xu D Galectin-3 is a negative regulator of lipopolysaccharide-mediated inflammation. J. Immunol., 2008-08-15;181(4):2781-9. 2008-08-15 [PMID: 18684969] (Bioassay) Bioassay
Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection. Am. J. Pathol., 2007-02-01;170(2):546-56. 2007-02-01 [PMID: 17255323] (Bioassay, Mouse) Bioassay Mouse
He J, Baum LG Presentation of galectin-1 by extracellular matrix triggers T cell death. J. Biol. Chem., 2003-11-14;279(6):4705-12. 2003-11-14 [PMID: 14617626] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol Lgals3
Uniprot