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Recombinant Mouse E-Cadherin Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse E-Cadherin Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 for this effect is 0.25-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse E-Cadherin protein
Asp157-Val709, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp157
Protein/Peptide Type
Recombinant Proteins
Gene
Cdh1
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-87 kDa, reducing conditions
Publications
Read Publications using
8875-EC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse E-Cadherin Protein, CF

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Nagafuchi, A. et al. (1987) Nature 329:341.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

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8875-EC
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Publications for E-Cadherin (8875-EC)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, Western Blot Control.


Filter By Application
Bioassay
(3)
Surface Plasmon Resonance
(1)
Western Blot Control
(1)
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Filter By Species
Mouse
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using 8875-EC Applications Species
DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody Proceedings of the National Academy of Sciences of the United States of America, 2022-10-18;119(43):e2209563119. 2022-10-18 [PMID: 36256815] (Western Blot Control, Mouse) Western Blot Control Mouse
X Zhou, M Yu, L Ma, J Fu, J Guo, J Lei, Z Fu, Y Fu, Q Zhang, CY Zhang, X Chen In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis Nature Communications, 2022-09-28;13(1):5700. 2022-09-28 [PMID: 36171212] (Bioassay, Mouse) Bioassay Mouse
M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension Nature Cell Biology, 2022-09-13;24(9):1341-1349. 2022-09-13 [PMID: 36100738] (Bioassay, Mouse) Bioassay Mouse
A Chaigne, MB Smith, RL Cavestany, E Hannezo, KJ Chalut, EK Paluch Three-dimensional geometry controls division symmetry in stem cell colonies Journal of Cell Science, 2021-01-01;0(0):. 2021-01-01 [PMID: 34160054] (Bioassay, Mouse) Bioassay Mouse
JM Segal, CM Ward Novel peptides for deciphering structural and signalling functions of E-cadherin in mouse embryonic stem cells Sci Rep, 2017-02-07;7(0):41827. 2017-02-07 [PMID: 28169326] (Surface Plasmon Resonance, Mouse) Surface Plasmon Resonance Mouse

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Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.
Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.
SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.
Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.
Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.
Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.
E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and ...  Read full blog post.
E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Bioinformatics

Gene Symbol Cdh1
Uniprot