Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Summary
Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 inhibits a constant dose of 100 ng/mL of
Recombinant
Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 30-180 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Dkk-1 protein Ser30-His272, with a C-terminal 10-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
27.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38 kDa and 41 kDa, reducing conditions
Publications
Read Publications using 1765-DK in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
dickkopf (Xenopus laevis) homolog 1
dickkopf homolog 1 (Xenopus laevis)
dickkopf related protein-1
Dickkopf-1
dickkopf-related protein 1
Dkk1
Dkk-1
hDkk-1
SKdickkopf-1 like
Background
Dickkopf
related protein 1 (Dkk-1) is the founding member of the Dickkopf family
of proteins that includes Dkk-1, -2, -3, -4, and a related protein,
Soggy (1, 2). Dkk proteins are secreted proteins that contain two
conserved cysteine-rich domains separated by a linker region. Each
domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a
40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino
acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1,
respectively. It also shares 41% and 36% aa identity with human Dkk-2
and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists
of the canonical Wnt signaling pathway (1, 2). This pathway is
activated by Wnt engagement of a receptor complex composed of the
Frizzled proteins and one of two low‑density lipoprotein
receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by
forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5).
Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate
Wnt signaling (4, 5). Dkk-1 is expressed throughout development and
antagonizes Wnt-7a during limb development (6, 7). Other sites of
expression include developing neurons, hair follicles and the retina of
the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition
is critical for bone formation and homeostasis (10). Insufficient or
excess Dkk-1 activity in bone results in increased or decreased bone
density, respectively (8, 11). In adults, Dkk-1 is expressed in
osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1
expression, which contributes to neuronal cell death (12).
Glinka, A. et al. (1998) Nature 391:357.
Niehrs, C. (2006) Oncogene 25:7469.
Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
Mao, B. et al. (2001) Nature 411:321.
Mao, B. et al. (2002) Nature 417:664.
Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
Adamska, M. et al. (2004) Dev. Biol. 272:134.
Li, J. et al. (2006) Bone 36:754.
Verani, R. et al. (2006) J. Neurochem. 101:242.
Pinzone, J.J. et al. (2009) Blood 113:517.
Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
The role of Wnts in neuroinflammation By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s... Read full blog post.
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