>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Publications
Read Publications using 9254-S9 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, TCEP and Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in water.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human S100A9 Protein, CF
CAGBMigration inhibitory factor-related protein 14
Calgranulin B
calgranulin-B
Calprotectin L1H subunit
CFAGMRP-14,60B8AG
CGLB
L1AG
LIAG
MAC387
MIF
MRP-14
MRP14Leukocyte L1 complex heavy chain
NIF
P14
protein S100-A9
S100 calcium binding protein A9 (calgranulin B)
S100 calcium binding protein A9
S100 calcium-binding protein A9 (calgranulin B)
S100 calcium-binding protein A9
S100A9
Background
S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium binding proteins (1, 2). It is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid (3-6). Human S100A9 contains two EF-hand calcium binding motifs and shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (7). S100A9
is a noncovalent homodimer that can also noncovalently heterodimerize with
S100A8; in the presence of calcium and zinc, the homodimer and heterodimers
will form tetramers (8-10). Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue which is not conserved in the mouse protein (11). Human S100A9 can also be modified by S-glutathionylation and oxidation (12). The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does (4). S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes (5, 6, 9, 13). S100A9 also promotes cartilage matrix destruction in osteoarthritis (14) as well as RAGE-dependent cardiac fibrosis and remodeling (6). In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide (15).
Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
Edgeworth, J. et al. (1991) J. Biol. Chem. 266:7706.
Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
Odink, K. et al. (1987) Nature 330:80.
Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
Ryckman, C. et al. (2003) J. Immunol. 170:3233.
Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
Edgeworth, J. et al. (1989) Nature 342:189.
Lim, S.Y. et al. (2010) J. Biol. Chem. 285:14377.
Simard, J.-C. et al. (2010) J. Leukoc. Biol. 87:905.
Schelbergen, R.F.P. et al. (2012) Arthritis Rheum. 64:1477.
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FAQs for S100A9 (9254-S9). (Showing 1 - 2 of 2 FAQs).
Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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