Recombinant Human S100A8/S100A9 Heterodimer Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two
EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, Flow Cytometry.

Showing Publications 1 - 10 of 10.

Publications using 8226-S8	Applications	Species
Chen, Q;Ishii, K;Mori, H;Nishijima, A;Arai, S;Miyazaki, T;Rosenthal, PB; Cryo-EM reveals structural basis for human AIM/CD5L recognition of polymeric immunoglobulin M Nature communications 2024-10-30 [PMID: 39477921] (Bioassay, Human)	Bioassay	Human
Basova, LV;Lindsey, A;McGovern, A;Rosander, A;Delorme-Walker, V;ElShamy, WM;Pendyala, VV;Gaskill, PJ;Ellis, RJ;Cherner, M;Iudicello, JE;Marcondes, MCG; MRP8/14 Is a Molecular Signature Triggered by Dopamine in HIV Latent Myeloid Targets That Increases HIV Transcription and Distinguishes HIV+ Methamphetamine Users with Detectable CSF Viral Load and Brain Pathology Viruses 2023-06-13 [PMID: 37376663] (Bioassay, Human)	Bioassay	Human
LGN de Almeida, D Young, L Chow, J Nicholas, A Lee, MC Poon, A Dufour, EO Agbani Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia Cells, 2022-04-07;11(8):. 2022-04-07 [PMID: 35455936] (Flow Cytometry, Human)	Flow Cytometry	Human
Z Li, O McGinn, Y Wu, A Bahreini, NM Priedigkei, K Ding, S Onkar, C Lampenfeld, CA Sartorius, L Miller, M Rosenzweig, O Cohen, N Wagle, JK Richer, WJ Muller, L Buluwela, S Ali, TC Bruno, DAA Vignali, Y Fang, L Zhu, GC Tseng, J Gertz, JM Atkinson, AV Lee, S Oesterreic ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation Nature Communications, 2022-04-19;13(1):2011. 2022-04-19 [PMID: 35440136] (Bioassay, Human)	Bioassay	Human
N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-09-14;36(11):109693. 2021-09-14 [PMID: 34525359] (Bioassay, Human)	Bioassay	Human
DG Bisson, K Sheng, S Kocabas, E Krock, A Teles, N Saran, JA Ouellet, L Haglund Toll-like receptor involvement in adolescent scoliotic facet joint degeneration J. Cell. Mol. Med., 2020-08-27;0(0):. 2020-08-27 [PMID: 32853438] (Bioassay, Human)	Bioassay	Human
V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020-05-12;31(6):107615. 2020-05-12 [PMID: 32402278] (Cell Culture, Human)	Cell Culture	Human
C Dubois, D Marcé, V Faivre, AC Lukaszewic, C Junot, F Fenaille, S Simon, F Becher, N Morel, D Payen High plasma level of S100A8/S100A9 and S100A12 at admission indicates a higher risk of death in septic shock patients Sci Rep, 2019-10-30;9(1):15660. 2019-10-30 [PMID: 31666644] (Bioassay, Human)	Bioassay	Human
J Sun, X Pan, LI Christians, XL Yuan, K Skovgaard, DEW Chatterton, SS Kaalund, F Gao, PT Sangild, S Pankratova Necrotizing enterocolitis is associated with acute brain responses in preterm pigs J Neuroinflammation, 2018-06-09;15(1):180. 2018-06-09 [PMID: 29885660] (Bioassay, Rat)	Bioassay	Rat
A Basu, S Munir, MA Mulaw, K Singh, B Herold, D Crisan, A Sindrilaru, N Treiber, M Wlaschek, M Huber-Lang, F Gebhard, K Scharffett A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing Sci Rep, 2018-04-18;8(1):6205. 2018-04-18 [PMID: 29670130] (Bioassay, Human)	Bioassay	Human

Array 8226-S8

• S100A8/S100A9 Heterodimer Antibodies
• S100A8/S100A9 Heterodimer ELISAs
• S100A8/S100A9 Heterodimer Proteins and Enzymes