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Recombinant Human LBP Protein

 (15 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
870-LP-025/CF
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Recombinant Human LBP Protein Summary

Details of Functionality
Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.25‑2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LBP protein
Ala26-Val481
Accession #
N-terminal Sequence
Ala26
Protein/Peptide Type
Recombinant Proteins
Gene
LBP
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-60 kDa, reducing conditions
Publications
Read Publications using
870-LP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LBP Protein

  • LBP
  • lipopolysaccharide binding protein
  • lipopolysaccharide-binding protein
  • LPS-binding protein
  • MGC22233

Background

Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.

  1. Hailman, E. et al. (1994) J. Exp. Med. 179:269.
  2. Jack, R.S. et al. (1997) Nature 389:742.
  3. Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.

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Recombinant Human
LBP Protein
870-LP
Recombinant Human LBP Protein
Species: Hu
Applications: Bioactivity

Publications for LBP (870-LP)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.


Filter By Application
Bioassay
(15)
Enzyme Assay
(1)
All Applications
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Human
(12)
Mouse
(1)
Chicken
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 870-LP Applications Species
Lin, YH;Kuo, NR;Shen, HC;Chang, YC;Lin, R;Liao, TL;Yeh, HY;Yang, YY;Hou, MC;Lin, HC; Prediction models combining zonulin, LPS, and LBP predict acute kidney injury and hepatorenal syndrome-acute kidney injury in cirrhotic patients Scientific reports 2023-08-11 [PMID: 37567912] (Bioassay, Human) Bioassay Human
A Pérez-Pons, M Jara-Aceve, A Henriques, P Navarro-Na, AC García-Mon, I Álvarez-Tw, CE Pedreira, L Sánchez-Mu, D Damasceno, C Caldas, JI Muñoz-Gonz, A Matito, J Flores-Mon, O González-L, I Criado, A Mayado, A Orfao Altered innate immune profile in blood of systemic mastocytosis patients Clinical and translational allergy, 2022-06-14;12(6):e12167. 2022-06-14 [PMID: 35734269] (Bioassay, Human) Bioassay Human
A Molinaro, A Koh, H Wu, M Schoeler, I Faggi, A Carreras, A Hallén, F Bäckhed, R Caesar Hepatic expression of Lipopolysaccharide Binding Protein (Lbp) is induced by the gut microbiota through Myd88 and impairs glucose tolerance in mice independent of obesity Mol Metab, 2020-04-16;0(0):100997. 2020-04-16 [PMID: 32305515] (Bioassay, Mouse) Bioassay Mouse
AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019-11-19;29(8):2505-2519.e4. 2019-11-19 [PMID: 31747616] (Bioassay, Human) Bioassay Human
SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018-03-05;0(0):. 2018-03-05 [PMID: 29360642] (Bioassay, Human) Bioassay Human
R Lehmann, MM Müller, TE Klassert, D Driesch, M Stock, A Heinrich, T Conrad, C Moore, UK Schier, R Guthke, H Slevogt Differential regulation of the transcriptomic and secretomic landscape of sensor and effector functions of human airway epithelial cells Mucosal Immunol, 2018-01-03;0(0):. 2018-01-03 [PMID: 29297499] (Bioassay, Human) Bioassay Human
PH Ding, RP Darveau, CY Wang, L Jin 3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes PLoS ONE, 2017-04-06;12(4):e0173223. 2017-04-06 [PMID: 28384159] (Bioassay, Human) Bioassay Human
B S Lashkari, DO Anumba Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4 PLoS ONE, 2017-03-15;12(3):e0173646. 2017-03-15 [PMID: 28296959] (Bioassay, Human) Bioassay Human
Annette C Wensink Granzymes A and K differentially potentiate LPS-induced cytokine response Cell Death Discov, 2016-12-12;2(0):16084. 2016-12-12 [PMID: 28028441] (Bioassay) Bioassay
Fang L, Xu Z, Wang G, Ji F, Mei C, Liu J, Wu G Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity. PLoS ONE, 2014-07-15;9(7):e101406. 2014-07-15 [PMID: 25025695] (Bioassay, Enzyme Assay, Human) Bioassay, Enzyme Assay Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol LBP
Uniprot