Novus Biologicals products are now on bio-techne.com

Recombinant Human IL-8/CXCL8 Protein, CF

Images

 
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL/CF) chemo­attracts the BaF3 mouse pro-B cell line transfected with human CXCR2. The ED50 for this effect is 0.5‑2.5 ng/mL.
1 μg/lane of Recombinant HumanIL-8/CXCL8 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-8/CXCL8 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human IL-8/CXCL8 protein
Ser28-Ser99
Accession #
N-terminal Sequence
Ser28
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL8
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
208-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-8/CXCL8 Protein, CF

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). IL-8/CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human IL-8/CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine IL-8/CXCL8 (4). There is no IL-8/CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human IL-8/CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of IL-8/CXCL8 is regulated by these truncations, by IL-8/CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). IL-8/CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, IL-8/CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). IL-8/CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8/CXCL8 interaction with CXCR1 (11). IL-8/CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).
  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Neote, K. et al. (1994) Blood 84:44.
  8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  9. Gerszten, R.E. et al. (1999) Nature 398:718.
  10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  14. Li, A. et al. (2005) Angiogenesis 8:63.
  15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
DCP00
Species: Hu
Applications: ELISA
DY417
Species: Mu
Applications: ELISA
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
201-LB
Species: Hu
Applications: BA
DVE00
Species: Hu
Applications: ELISA
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
DRN00B
Species: Hu
Applications: ELISA
DY453
Species: Mu
Applications: ELISA
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
6507-IL/CF
Species: Hu
Applications: BA
7954-GM/CF
Species: Hu
Applications: BA
DY1707
Species: Hu
Applications: ELISA
NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
202-IL
Species: Hu
Applications: BA

Publications for CXCL8/IL-8 (208-IL/CF)(123)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Virus, Zebrafish.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), Hemofiltration Procedure, In Vivo.


Filter By Application
Binding Assay
(4)
Bioassay
(89)
Cell Culture
(4)
ELISA (Standard)
(20)
Hemofiltration Procedure
(1)
In Vivo
(6)
All Applications
Filter By Species
Human
(89)
Mouse
(8)
Rat
(2)
Bovine
(1)
Porcine
(1)
Virus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using 208-IL/CF Applications Species
LJ Pallett, L Swadling, M Diniz, AA Maini, M Schwabenla, AD Gasull, J Davies, S Kucykowicz, JK Skelton, N Thomas, NM Schmidt, OE Amin, US Gill, KA Stegmann, AR Burton, E Stephenson, G Reynolds, M Whelan, J Sanchez, R de Maeyer, C Thakker, K Suveizdyte, I Uddin, AM Ortega-Pri, C Grant, F Froghi, G Fusai, S Lens, S Pérez-Del-, W Al-Akkad, G Mazza, M Noursadegh, A Akbar, PTF Kennedy, BR Davidson, M Prinz, BM Chain, M Haniffa, DW Gilroy, M Dorner, B Bengsch, A Schurich, MK Maini Tissue CD14+CD8+ T cells reprogrammed by myeloid cells and modulated by LPS Nature, 2023-01-25;614(7947):334-342. 2023-01-25 [PMID: 36697826] (Bioassay, Human) Bioassay Human
R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance Oncogene, 2022-07-19;40(3):111097. 2022-07-19 [PMID: 35858551] (Bioassay, Human) Bioassay Human
L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo Cell Reports, 2022-05-31;39(9):110876. 2022-05-31 [PMID: 35649374] (Bioassay, Human) Bioassay Human
A Conod, M Silvano, A Ruiz I Alt On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm Cell Reports, 2022-03-08;38(10):110490. 2022-03-08 [PMID: 35263600] (Bioassay, Human) Bioassay Human
ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling Journal of leukocyte biology, 2022-02-08;0(0):. 2022-02-08 [PMID: 35137444] (Bioassay, Mouse) Bioassay Mouse
M Lesouhaiti, M Gregoire, A Gacouin, V Coirier, A Frerou, C Piau, V Cattoir, E Dumontet, M Revest, P Tattevin, A Roisne, JP Verhoye, E Flecher, Y Le Tulzo, K Tarte, JM Tadié Neutrophil function and bactericidal activity against Staphylococcus aureus after cardiac surgery with cardiopulmonary bypass Journal of leukocyte biology, 2021-08-23;0(0):. 2021-08-23 [PMID: 34425029] (Bioassay, Human) Bioassay Human
J Lee, JA Choi, HH Ju, JE Kim, SY Paik, PV Rao Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells Scientific Reports, 2021-07-22;11(1):14950. 2021-07-22 [PMID: 34294770] (Bioassay, Human) Bioassay Human
N Kashiwagi, F Saito, H Maegawa, K Kaneda Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results Cytokine, 2021-01-01;139(0):155410. 2021-01-01 [PMID: 33395614] (Bioassay, Human) Bioassay Human
SJ Evani, SLR Karna, J Seshu, KP Leung Pirfenidone regulates LPS mediated activation of neutrophils Sci Rep, 2020-11-17;10(1):19936. 2020-11-17 [PMID: 33203891] (Bioassay, Human) Bioassay Human
L Rankin, S Gouveia-Fi, KP Danielsson, CJ Fowler Relative Deficiency of Anti-Inflammatory N-Acylethanolamines Compared to Prostaglandins in Oral Lichen Planus Biomedicines, 2020-11-06;8(11):. 2020-11-06 [PMID: 33172176] (Cell Culture, Human) Cell Culture Human
Show All 123 Publications.

Reviews for CXCL8/IL-8 (208-IL/CF) (0)

There are no reviews for CXCL8/IL-8 (208-IL/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CXCL8/IL-8 (208-IL/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-8/CXCL8 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CXCL8
Uniprot