Recombinant Human IL-6 (HEK293-expressed) Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA Capture, In Vivo.

Showing Publications 1 - 8 of 8.

Publications using 7270-IL/CF	Applications	Species
Choi, BR;Johnson, KR;Maric, D;McGavern, DB; Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature Nature immunology 2023-05-29 [PMID: 37248420] (In Vivo, Mouse)	In Vivo	Mouse
Ma, HY;Li, Q;Wong, WR;N'Diaye, EN;Caplazi, P;Bender, H;Huang, Z;Arlantico, A;Jeet, S;Wong, A;Emson, C;Brightbill, H;Tam, L;Newman, R;Roose-Girma, M;Sandoval, W;Ding, N; LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung Science advances 2023-05-26 [PMID: 37235663] (Bioassay, Human)	Bioassay	Human
RD de Ruiter, LE Wisse, T Schoenmake, M Yaqub, G Sánchez-Du, EMW Eekhoff, D Micha TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva International Journal of Molecular Sciences, 2023-01-24;24(3):. 2023-01-24 [PMID: 36768622] (Bioassay, Human)	Bioassay	Human
Z Cai, S Tian, T Klein, L Tu, LW Geenen, T Koudstaal, AE van den Bo, YB de Rijke, IKM Reiss, E Boersma, C van der Le, M Van Faasse, I Kema, DJ Duncker, KA Boomars, K Tran-Lundm, C Guignabert, D Merkus Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha Scientific Reports, 2022-07-19;12(1):12326. 2022-07-19 [PMID: 35853948] (Bioassay, Human)	Bioassay	Human
T Manshouri, I Veletic, P Li, CC Yin, SM Post, S Verstovsek, Z Estrov GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes Cell Death & Disease, 2022-05-20;13(5):481. 2022-05-20 [PMID: 35595725] (Bioassay, Xenograft)	Bioassay	Xenograft
AK Ajay, L Zhao, S Vig, M Fujiwara, S Thakurela, S Jadhav, A Cho, IJ Chiu, Y Ding, K Ramachandr, A Mithal, A Bhatt, P Chaluvadi, MK Gupta, SI Shah, VS Sabbisetti, AM Waaga-Gass, DA Frank, G Murugaiyan, JV Bonventre, LL Hsiao Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration Cell Reports, 2022-03-08;38(10):110473. 2022-03-08 [PMID: 35263586] (Bioassay, Human)	Bioassay	Human
S Jadhav, S Tripathi, A Chandrekar, SS Waikar, LL Hsiao A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 2021-01-22;16(1):e0245614. 2021-01-22 [PMID: 33481901] (ELISA Capture, Human)	ELISA Capture	Human
Y Wada, K Tokuda, Y Morine, S Okikawa, S Yamashita, T Ikemoto, S Imura, Y Saito, S Yamada, M Shimada The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment Oncotarget, 2020-12-08;11(49):4593-4604. 2020-12-08 [PMID: 33346211] (Cell Culture, Human)	Cell Culture	Human

Array 7270-IL/CF

• IL-6 Antibodies
• IL-6 Antibody Pair
• IL-6 ELISA Kits
• IL-6 Lysate
• IL-6 Proteins
• IL-6 Proteins and Enzymes
• IL-6 ELISAs