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Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

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Recombinant Human IFN-gamma (Catalog # 10067‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.06‑0.36 ng/mL
Recombinant Human IFN-gamma (Catalog # 10067‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this ...read more
2 μg/lane of Recombinant Human IFN‑ gamma (HEK293-expressed) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 19-25 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
10067-IF-100
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Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.06-0.36 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IFN-gamma protein
Met1-Gln166
Accession #
N-terminal Sequence

Gln24 inferred from enzymatic pyroglutamate treatment revealing Asp25.
Structure / Form
Noncovalent homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19-25 kDa, reducing conditions
Publications
Read Publication using
10067-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance (4, 5). The mature protein shares 36% and 35% amino acid (aa) sequence identity with mouse and rat IFN-gamma, respectively. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (6, 7). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (8). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (8, 9). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (10, 11). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (9).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Sareneva, T. et al. (1994) J. Biochem. 303:831.
  5. Sareneva, T. et al. (1995) J. Biochem. 308:9.
  6. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  7. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  8. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  9. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  10. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  11. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Recombinant Human
IFN-gamma (HEK293-exp ...
10067-IF
Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF
Species: Hu
Applications: Bioactivity

Publications for IFN-gamma (10067-IF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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