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Recombinant Human GIF Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human GIF Protein, CF Summary

Details of Functionality
Bioassay data are not available.
Source
Human embryonic kidney cell, HEK293-derived human GIF protein
Ser19-Tyr417 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
GIF
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
44-60 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GIF Protein, CF

  • gastric intrinsic factor (vitamin B synthesis)
  • GIF
  • IF
  • IFIntrinsic factor
  • IFMH
  • IFMHgastric intrinsic factor
  • INF
  • TCN3

Background

Gastric Intrinsic Factor (GIF) also known as Intrinsic Factor (IF) is a 45-55 kDa glycoprotein that is secreted by parietal cells of the gastric mucosa. GIF is a member of the eukaryotic cobalamin (Cbl or Vitamin B12) family of transport proteins that mediate the uptake of cobalamin by intestinal epithelial cells lining the ileum (1). GIF comprises two domains, an alpha and a beta -domain containing approximatively 270 and 110 residues respectively (2). Cobalamin binds to GIF at the interface of the alpha  and beta  domains (2). The Cbl-GIF complex can then interact with its specific receptor, Cubilin, through the amino-terminal portion of GIF (3). This complex associates with Amnionless (AMN) which directs subcellular localization and endocytosis of Cubilin and its ligands (4). Homozygous nonsense and missense mutations in the GIF gene have been linked to hereditary juvenile megaloblastic anemia. These patients show nutritional cobalamin insufficiency due to its intestinal malabsorption (5). Mature human GIF shares 82% and 79% amino acid sequence identity with mouse GIF and rat GIF respectively.
  1. Hewitt, J.E. et al. (1991) Genomics 10:432.
  2. Mathews, F.S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:17311.
  3. Tang, L-H. et al. (1992) J. Biol. Chem. 267:22982.
  4. Fyfe, J.C. et al. (2004) Blood 103:1573.
  5. Tanner, S.M. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4130.

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Bioinformatics

Gene Symbol GIF
Uniprot