Recombinant Human Dkk-1 N-Terminal Fragment Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. When Recombinant Human Dkk‑1 N-Terminal Fragment is coated at 1 μg/mL, Mouse CKAP4/p63
(Catalog #
9734-CK)
binds with an ED 50 of 9-54 ng/mL. |
Source |
Chinese Hamster Ovary cell line, CHO-derived human Dkk-1 protein Thr32-Ser141, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
Thr32, Ser35 & Ser39
|
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
13 - 21 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Dkk-1 N-Terminal Fragment Protein, CF
Background
Dickkopf related protein 1 (Dkk-1) is
the founding member of the Dickkopf family of proteins that includes Dkk-1, -2,
-3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins
that contain two conserved cysteine-rich domains (CRDs), CRD1 (N-terminal) and CRD2 (C-terminal), separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature human Dkk-1 is
a 40 kDa glycosylated protein. Within the N-terminal fragment [amino acids (aa) 32-141] that includes the CRD1, human DKK-1 shares 85% aa sequence identity with mouse Dkk-1. Dkk-1 antagonizes Wnt/beta-catenin signaling, an activity
for which the C-terminal CRD2 is both necessary and sufficient (4, 5),
while the N-terminal CRD1 was required for binding to CKAP4 (6).
However,
crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a
bipartite inhibitor, with both CRDs binding the extracellular domain of
LRP-6
simultaneously (7-9). Dkk-1/LRP-6/Krm2 complex
internalization has been shown to down-regulate Wnt signaling (4, 10). Dkk-1 is
expressed throughout development and antagonizes Wnt-7a during limb development
(11, 12). Other sites of expression include developing neurons, hair follicles,
and the retina (13, 14). The balance between Wnt signaling and Dkk-1 inhibition
is critical for bone formation and homeostasis (15). Insufficient or excess
Dkk-1 activity in bone results in increased or decreased bone density,
respectively (13, 16). In adults, Dkk-1 is expressed in osteoblasts and
osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which
contributes to neuronal cell death (17). Dkk-1 also likely has a complex role in
cancer, as it has been shown to act as a tumor suppressor and also to promote
metastasis (18-20).
- Krupnik, V.E. et al. (1999) Gene 238:301.
- Niehrs, C. (2006) Oncogene 25:7469.
- Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
- Mao, B. et al. (2001) Nature 411:321.
- Brott, B.K. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
- Kimura, H. et al. (2016) J. Clin. Invest. 126:7.
- Chen, S. et al. (2011) Dev. Cell 21:848.
- Bourhis, E. et al. (2011) Structure 19:1433.
- Cheng, Z. et al. (2011) Nat. Struct. Mol. Biol. 18:1204.
- Mao, B. et al. (2002) Nature 417:664.
- Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
- Adamska, M. et al. (2004) Dev. Biol. 272:134.
- Li, J. et al. (2006) Bone 39:754.
- Verani, R. et al. (2007) J. Neurochem. 100:242.
- Pinzone, J.J. et al. (2009) Blood 113:517.
- Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
- Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
- Menezes, M.E. et al. (2012) Int. J. Cancer 130:1477.
- Li, S. et al. (2013) PLoS One 8:e84944.
- Chen, L. et al. (2013) Mol. Cancer 12:157.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu
Applications: WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA, BA
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: CyTOF-ready, Flow, ICC
Species: Hu
Applications: BA
Species: Mu
Applications: BA
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ELISA, IHC
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: BA, BA
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: BA
Species: Hu
Applications: Bioactivity
Publications for Dkk-1 (9837-DK) (0)
There are no publications for Dkk-1 (9837-DK).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Dkk-1 (9837-DK) (0)
There are no reviews for Dkk-1 (9837-DK).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for Dkk-1 (9837-DK) (0)