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Recombinant Human Annexin A6 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Human Annexin A6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhAnnexin-A6 can bind rhDecorin with an apparent KD <2 nM.
Source
E. coli-derived human Annexin A6 protein
Ala2-Asp673, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
ANXA6
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
76.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Annexin A6 Protein, CF

  • 67 kDa calelectrin
  • Annexin A6
  • annexin VI (p68)
  • Annexin VI
  • Annexin-6
  • ANX6
  • ANXA6
  • calcium-binding protein p68
  • Calelectrin
  • calphobindin II
  • calphobindin-II
  • CBP68
  • chromobindin-20
  • CPB-II
  • CPB-II
  • Lipocortin VI
  • p68
  • p70
  • Protein III

Background

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60 - 70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5 - 7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

  1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
  2. Crompton, M.R. et al. (1988) EMBO J. 7:21.
  3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
  4. Grewal, T. et al. (2006) Bioessays 28:1211.
  5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
  6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
  7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
  8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

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Bioinformatics

Gene Symbol ANXA6
Uniprot