Recombinant Cynomolgus/Rhesus Macaque Dkk-1 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature Cynomolgus/rhesus Dkk-1 is a 40 kDa glycosylated protein that shares 99% amino acid sequence identity with human Dkk-1. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). The C-terminal cysteine-rich domain 2 (CRD2) of Dkk-1 has been shown to interact with LRP5 or LRP6 (5). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 6). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 6). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (7, 8). Other sites of expression include developing neurons, hair follicles and the retina of the eye (9, 10). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (11). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (9, 12). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (13).

Array 10450-DK

• Dkk-1 Antibodies
• Dkk-1 Antibody Pair
• Dkk-1 ELISA Kit
• Dkk-1 Lysate
• Dkk-1 Proteins and Enzymes
• Dkk-1 ELISAs