Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1).  In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, cynomolgus/rhesus GPC3 shares 99% amino acid sequence identity with human GPC3.  GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5).  Our Avi-tag Biotinylated Recombinant  Glypican 3  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Array 10509-GP

• Glypican 3 Antibodies
• Glypican 3 ELISA Kits
• Glypican 3 Lysate
• Glypican 3 Protein
• Glypican 3 ELISAs
• Glypican 3 Proteins and Enzymes