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Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

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Recombinant Cynomolgus Monkey IL‑18 BPa inhibits Recombinant Human IL-18-induced IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL.
Recombinant Cynomolgus Monkey IL‑18 BPa inhibits Recombinant Human IL-18-induced IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL.
2 μg/lane of Recombinant Cynomolgus Monkey IL‑18 BPa was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-60 kDa.

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Bioactivity
Format
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Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-18 BPa protein
Thr28-Pro207, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Thr28
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
54-60 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

  • IL18 BPa
  • IL-18 BPa
  • IL18BP
  • IL-18BP
  • IL18BPa
  • interleukin 18 binding protein
  • interleukin-18-binding protein
  • MC51L-53L-54L homolog gene product
  • tadekinig-alfa

Background

Cynomolgus Interleukin 18 binding protein (IL-18 BP) is a 20 kDa secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity (1). IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. Human IL-18BP encodes for at least four isoforms by alternative splicing. The IL-18 BP isoform a and c each contains one immunoglobulin (Ig)-like C2-type domain while isoform b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins (2). Cynomolgus IL-18BPa shares 83% sequence identity with human and 62% with mouse IL-18BPa, respectively. Several poxviruses also encode proteins with sequence similarity to IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP (3).

  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.

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