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RCN1 Antibody

Images

 
Independent Antibodies: Western Blot: RCN1 Antibody [NBP1-83483] - Analysis using Anti-RCN1 antibody NBP1-83483 (A) shows similar pattern to independent antibody NBP2-37987 (B).
Immunocytochemistry/ Immunofluorescence: RCN1 Antibody [NBP1-83483] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RCN1 Antibody [NBP1-83483] - Staining of human pancreas shows very weak cytoplasmic positivity in intercalated ducts and Islets of Langerhans.
Western Blot: RCN1 Antibody [NBP1-83483] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: RCN1 Antibody [NBP1-83483] - Staining of human small intestine shows moderate cytoplasmic positivity in Paneth cells
Immunohistochemistry-Paraffin: RCN1 Antibody [NBP1-83483] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RCN1 Antibody [NBP1-83483] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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RCN1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGKIVDRIDNDGDGFVTTEELKTWIKRVQKRYIFDNVAKVWKDYDRDKDDKISWEEYKQA
Predicted Species
Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RCN1 Protein (NBP1-83483PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RCN1 Antibody

  • FLJ37041
  • FLJ55835
  • PIG20
  • proliferation-inducing gene 20
  • RCAL
  • RCN
  • reticulocalbin 1, EF-hand calcium binding domain
  • reticulocalbin-1

Background

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RCN1 Antibody (NBP1-83483) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for RCN1 Antibody (NBP1-83483)

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Blogs on RCN1

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Bioinformatics

Gene Symbol RCN1