![Western Blot: RCN1 Antibody [NB500-256] - Detection of human and mouse Reticulocalbin-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RCN1-Antibody-Western-Blot-NB500-256-img0005.jpg "Western Blot: RCN1 Antibody [NB500-256] - Detection of human and mouse Reticulocalbin-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide that maps to a region between residues 1 and 75 of human Reticulocalbin-1, EF-hand calcium binding domain using the numbering given in Swiss-Prot entry Q15293 (GeneID 5954). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RCN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for RCN1 Antibody (NB500-256)Find related products by research area.
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![Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 (NB500-256). Detection: DAB](http://images.novusbio.com/fullsize/RCN1-Antibody-Immunohistochemistry-NB500-256-img0006.jpg "Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 (NB500-256). Detection: DAB")
![Immunoprecipitation: RCN1 Antibody [NB500-256] - Detection of human Reticulocalbin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 (lot NB500-256-3) used for IP at 6 ug per reaction. Reticulocalbin-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-256-2). For blotting immunoprecipitated Reticulocalbin-1, NB500-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RCN1-Antibody-Immunoprecipitation-NB500-256-img0007.jpg "Immunoprecipitation: RCN1 Antibody [NB500-256] - Detection of human Reticulocalbin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 (lot NB500-256-3) used for IP at 6 ug per reaction. Reticulocalbin-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-256-2). For blotting immunoprecipitated Reticulocalbin-1, NB500-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")