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Human RAR alpha/NR1B1 ELISA Kit (Colorimetric)

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ELISA: Human RAR alpha/NR1B1 ELISA Kit (Colorimetric) [NBP3-00419] - Samples were spiked with high concentrations of Human RAR alpha/NR1B1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human RAR alpha/NR1B1 ELISA Kit (Colorimetric) [NBP3-00419] - Samples were spiked with high concentrations of Human RAR alpha/NR1B1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human RAR alpha/NR1B1 ELISA Kit (Colorimetric) [NBP3-00419] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

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Human RAR alpha/NR1B1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human RAR alpha/NR1B1 in samples. No significant cross-reactivity or interference between Human RAR alpha/NR1B1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.78%
Intra-Assay
4.47%
Spike Recovery
86 - 107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
RARA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human RAR alpha/NR1B1 ELISA Kit (Colorimetric)

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human RAR alpha/NR1B1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol RARA