RANK/TNFRSF11A Antibody (9A725) [PE]

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow Cytometry Control, Flow-CS, IHC-P, WB.

Showing Publications 1 - 10 of 10.

Publications using NB100-56029	Applications	Species
Lafferty MK, Fantry L, Bryant J et al. Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats Pathog Dis 2013-12-09 [PMID: 24376119] (Flow-CS, Rat) Details: This citation used the PE version of this antibody.	Flow-CS	Rat
Izawa T, Zou W, Chappel JC et al. c-Src links a RANK/avB3 integrin complex to the osteoclast cytoskeleton. Mol Cell Biol. 2012-07-01 [PMID: 22615494] (WB, Mouse) Details: RANk (IMG-128A). 1. WB: Mice Splenocytes, Figure 1, 2C, 2D, & 2E.	WB	Mouse
Riegel A, Maurer T, Prior B et al. Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL. Eur J Immunol. 2012-04-01 [PMID: 22531921] (Flow Cytometry Control, Flow-CS) Details: Antibody cited: RANK-PE, clone 9A725 (IMG-128D) for Flow (cell surface) + Flow (intracellular): 1. Whole blood and isolated polymorhonuclear neutrophils (PMNs): Figs 1, 3, 4, S1-S3. 3BS1, S2, polymorphonuclear neutrophils (PMNs) Notes: 1. RANK expression	Flow Cytometry Control, Flow-CS
Huang G, Wen Q, Gao Q et al. An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples. Biotechnol Lett. 2011-10-01 [PMID: 21660576] Details: Antibody cited: RANK, clone 9A725 (IMG-128A). 1. WB: Fig 4b (RANK-transfected A549 cells). Recombinantly expressed RANK was dected at 97 kDa. A549 transfected with an empty plasmid were used as a negative control as A549 cells lack endogenous expression o
Crockett JC, Mellis DJ, Shennan KI et al. Signal peptide mutations in RANK prevent downstream activation of NF-kB. J Bone Miner Res. 2011-08-01 [PMID: 21472776] Details: RANk/TNF11aR/ODFR (IMG-128A). WB: 293 cells transfected with RANk, Fig 1. Note: The specificityof the RANk antibody (IMG-128A) was transfected validated by WB in Rank transfected 293 cell line, Fig 1.
Ayon Haro ER, Ukai T, Yokoyama M et al. Locally administered interferon-gamma accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation. J Periodontal Res. 2011-06-01 [PMID: 21361961] (Flow-CS, Mouse) Details: RANK-PE, clone 9A725 (IMG-128D). Flow (cell surface): Primary mouse bone marrow macrophages. By flow cytometry analysis, ~95% of the bone marrow macrophage population was positive for RANK and CD11b (data described on page 363). Note: The RANK-PE mAb was used to help characterize the primary bone macrophages isolated from mouse tibiae and femurs (see Material and Methods, page 363).	Flow-CS	Mouse
Cannon JG, Kraj B, Sloan G. Follicle-stimulating hormone promotes RANK expression on human monocytes. Cytokine. 2011-02-01 [PMID: 21159522] (Flow Cytometry Control, Human)	Flow Cytometry Control	Human
Lee B, Kim TH, Jun JB et al. Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta. J Immunol. 2010-11-15 [PMID: 20935210] (Human) Details: RANk, clone 9A725 (IMG-128A). WB: Fig 2C (primary human monocyte-derived preosteoclasts). RANk was detected at 97 kDa. IL-1B treatment down regulated RANk expression.		Human
Bharti AC, Takada Y, Shishodia S, Aggarwal BB. Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J Biol Chem. 2004-02-13 [PMID: 14645259] (WB, Flow-CS)	WB, Flow-CS
Fiumara P, Snell V, Li Y et al. Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines. Blood. 2001-11-01 [PMID: 11675352] (IHC-P, WB)	IHC-P, WB

Array NB100-56029

• RANK/TNFRSF11A Antibodies
• RANK/TNFRSF11A ELISA Kits
• RANK/TNFRSF11A Lysate
• RANK/TNFRSF11A Proteins
• RANK/TNFRSF11A ELISA
• RANK/TNFRSF11A Proteins and Enzymes