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RanGAP1 Antibody (OTI1B4) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73797AF350
Alexa Fluor 405
NBP2-73797AF405
Alexa Fluor 488
NBP2-73797AF488
Alexa Fluor 532
NBP2-73797AF532
Alexa Fluor 594
NBP2-73797AF594
Alexa Fluor 647
NBP2-73797AF647
Alexa Fluor 700
NBP2-73797AF700
Alexa Fluor 750
NBP2-73797AF750
Allophycocyanin
NBP2-73797APC
Biotin
NBP2-73797B
CoraFluor 1
NBP2-73797CL1
DyLight 350
NBP2-73797UV
DyLight 488
NBP2-73797G
DyLight 550
NBP2-73797R
DyLight 594
NBP2-73797DL594
DyLight 650
NBP2-73797C
DyLight 680
NBP2-73797FR
DyLight 755
NBP2-73797IR
FITC
NBP2-73797F
HRP
NBP2-73797H
Janelia Fluor 525
NBP2-73797JF525
Janelia Fluor 549
NBP2-73797JF549
Janelia Fluor 585
NBP2-73797JF585
Janelia Fluor 635
NBP2-73797JF635
Janelia Fluor 646
NBP2-73797JF646
Janelia Fluor 669
NBP2-73797JF669
PE
NBP2-73797PE
PerCP
NBP2-73797PCP
Unconjugated
NBP2-02623
mFluor Violet 450 SE
NBP2-73797MFV450
mFluor Violet 500 SE
NBP2-73797MFV500
mFluor Violet 610 SE
NBP2-73797MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02623
Azide and BSA Free
NBP2-73797
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RanGAP1 Antibody (OTI1B4) [DyLight 405] Summary

Immunogen
Full length recombinant protein of human RanGAP1 (NP_002874) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RANGAP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for RanGAP1 Antibody (OTI1B4) [DyLight 405]

  • Fug1
  • KIAA1835Fug1
  • MGC20266
  • Ran GTPase activating protein 1
  • ran GTPase-activating protein 1
  • RanGAP1
  • SD
  • segregation distorter homolog (Drosophila)
  • segregation distorter homolog
  • segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RANGAP1