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Rad51 Antibody (S04-3C6)

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Western Blot: Rad51 Antibody (S04-3C6) [NBP3-14968] - Western blot detection of Rad51 in A549, HL-60, U2OS cell lysates using Rad51 Rabbit mAb (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 37kDa.
Immunohistochemistry-Paraffin: Rad51 Antibody (S04-3C6) [NBP3-14968] - Immunohistochemistry of Rad51 in paraffin-embedded Human tonsil using Rad51 Rabbit mAb at dilution 1/50

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
S04-3C6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rad51 Antibody (S04-3C6) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Rad51 (Uniprot#: Q06609)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RAD51
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for Rad51 Antibody (S04-3C6)

  • BRCC5
  • HRAD51
  • HsRad51
  • HsT16930
  • RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • RAD51 homolog A
  • Rad51
  • RAD51ABRCA1/BRCA2-containing complex, subunit 5
  • RAD51L3
  • RecA, E. coli, homolog of
  • RECADNA repair protein RAD51 homolog 1
  • RecA-like protein
  • recombination protein A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Rad51 Antibody (NBP3-14968)

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Blogs on Rad51.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

RAD51: The cell's 'Mr. Fix-it'
RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Determining DMC1's role in Homologus Recombination
The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb...  Read full blog post.

Rad51 Antibody Reveals a Canine Model for Human Breast Cancer
Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb...  Read full blog post.

Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research
Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con...  Read full blog post.

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Bioinformatics

Gene Symbol RAD51