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RAD18 Antibody (79B1048.1) - Azide Free

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Western Blot: RAD18 Antibody (79B1048.1) - Azide Free [NBP2-33217] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate. Image from the standard format of this antibody.
Western Blot: RAD18 Antibody (79B1048.1) - Azide Free [NBP2-33217] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate. Image from the standard format of this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
79B1048.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56523AF350
Alexa Fluor 405
NB100-56523AF405
Alexa Fluor 488
NB100-56523AF488
Alexa Fluor 532
NB100-56523AF532
Alexa Fluor 594
NB100-56523AF594
Alexa Fluor 647
NB100-56523AF647
Alexa Fluor 700
NB100-56523AF700
Alexa Fluor 750
NB100-56523AF750
Biotin
NB100-56523B
CoraFluor 1
NB100-56523CL1
DyLight 350
NB100-56523UV
DyLight 405
NB100-56523V
DyLight 488
NB100-56523G
DyLight 550
NB100-56523R
DyLight 594
NB100-56523DL594
DyLight 650
NB100-56523C
DyLight 680
NB100-56523FR
DyLight 755
NB100-56523IR
FITC
NB100-56523F
HRP
NB100-56523H
Janelia Fluor 525
NB100-56523JF525
Janelia Fluor 549
NB100-56523JF549
Janelia Fluor 585
NB100-56523JF585
Janelia Fluor 635
NB100-56523JF635
Janelia Fluor 646
NB100-56523JF646
Janelia Fluor 669
NB100-56523JF669
Unconjugated
NB100-56523
mFluor Violet 450 SE
NB100-56523MFV450
mFluor Violet 500 SE
NB100-56523MFV500
mFluor Violet 610 SE
NB100-56523MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56523
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RAD18 Antibody (79B1048.1) - Azide Free Summary

Immunogen
The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD18
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
Application Notes
In HL60, a 54 kDa band should be detected.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin. Does not contain BSA
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RAD18 Antibody (79B1048.1) - Azide Free

  • EC 6.3.2.-
  • hHR18
  • hRAD18
  • postreplication repair protein hRAD18p
  • Postreplication repair protein RAD18
  • RAD18 homolog (S. cerevisiae)
  • RAD18, S. cerevisiae, homolog
  • RING finger protein 73
  • RNF73E3 ubiquitin-protein ligase RAD18

Background

RAD18 is encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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RAD18 Antibody
(79B1048.1) - Azide F
NBP2-33217
RAD18 Antibody (79B1048.1) - Azide Free
Species: Hu
Applications: WB
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD18