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Rab3D Antibody

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Immunocytochemistry/ Immunofluorescence: Rab3D Antibody [NB120-3337] - IF analysis of RAB3D was performed using 70% confluent log phase 3T3-L1 cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Rab3D Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D.
Specificity
Detects recombinant mouse rab 3D. This does not detect rab 3A, 3B, or 3C.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB3D
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 2.0 ug/ml
Application Notes
WB: Detects an approx. 29 kDa protein representing recombinant mouse Rab 3D.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Rab3D Antibody

  • D2-2
  • GOVras-related protein Rab-3D
  • RAB16glioblastoma overexpressed
  • RAB3D, member RAS oncogene family
  • RAD3DRab3D upregulated with myeloid differentiation

Background

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab3D Antibody (NB120-3337) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAB3D
Entrez