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RAB27B Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining in human prostate and tonsil tissues . Corresponding RAB27B RNA-seq data are presented for the same tissues.
Western Blot: RAB27B Antibody [NBP1-89538] - Analysis in control (vector only transfected HEK293T lysate) and RAB27B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human prostate shows strong apical membrane and cytoplasmic positivity in glandular cells.
Western Blot: RAB27B Antibody [NBP1-89538] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.
Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human tonsil shows mainly very weak positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

RAB27B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FETSAATGQNVEKAVETLLDLIMKRMEQCVEKTQIPDTVNGGNSGNLDGEKPPEKKCIC
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB27B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RAB27B Protein (NBP1-89538PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RAB27B Antibody

  • C25KG
  • RAB27B, member RAS oncogene family
  • ras-related protein Rab-27B

Background

RAB27B may be involved in targeting uroplakins to urothelial apical membranes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAB27B