![Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and Rab3D showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing Rab3D.. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764). Courtesy of: Dario Alessi Lab, University of Dundee.](http://images.novusbio.com/fullsize/Rab3D-Antibody-5E9-Western-Blot-NBP3-14764-img0001.jpg "Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and Rab3D showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing Rab3D.. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764). Courtesy of: Dario Alessi Lab, University of Dundee.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | 5E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant Rab3D protein (Accession #NP_004274) |
| Localization | Membrane |
| Specificity | Detects ~24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAB3D |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | PBS pH 7.4, 50% glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | RAB3D |
![Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney.. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Rab3D-Antibody-5E9-Western-Blot-NBP3-14764-img0002.jpg "Western Blot: Rab3D Antibody (5E9) [NBP3-14764] - Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of Rab3D protein using Mouse Anti-Rab3D Monoclonal Antibody, Clone 5E9 (NBP3-14764). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney.. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rab3D Monoclonal Antibody (NBP3-14764) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")