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Rab13 Antibody

Images

 
Genetic Strategies: Knockdown Validated: Rab13 Antibody [NBP1-85799] - Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 ...read more
Genetic Strategies: Knockdown Validated: Rab13 Antibody [NBP1-85799] - Cells treated with the indicated PMOs or siRNAs were analyzed by Western blot (upper panels) to detect RAB13 and GAPDH protein levels. ...read more
Genetic Strategies: Western Blot: Rab13 Antibody [NBP1-85799] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB13 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Rab13 Antibody [NBP1-85799] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human prostate shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemical staining of CST1 in nasal epithelial cells.Representative immunostaining of CST1 expression inthe nasal epithelium of 2 SAR patients (A and B) and 2 Control subjects (C and D). Magnification:×200. ...read more
Primary human grade III astrocytoma xenotransplant.(A) T2-weighted MRI (coronal) visualizes advanced tumor in the right hemisphere of the brain. (B) Correlative dynamic PET image (coronal) of same advanced tumor in the ...read more
Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB and expression of BDNF between groups in the PFC and hippocampus (n = 3-4). (d, e) pCREB and BDNF ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Func, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Rab13 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCS
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Proximity Ligation Assay Reported in scientific literature (PMID:31290739)
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Rab13 Protein (NBP1-85799PEP)
Publications
Read Publications using
NBP1-85799 in the following applications:

  • 1 publication
  • PLA
    1 publication
  • WB
    3 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:31290739).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rab13 Antibody

  • Cell growth-inhibiting gene 4 protein
  • growth-inhibiting gene 4 protein
  • Rab13
  • RAB13, member RAS oncogene family
  • RAS-associated protein RAB13
  • ras-related protein Rab-13

Background

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab13 Antibody (NBP1-85799)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, PLA, WB.


Filter By Application
ICC/IF
(1)
PLA
(1)
WB
(3)
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Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab13 Antibody (NBP1-85799) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Rab13

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Bioinformatics

Gene Symbol RAB13