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Rab13 Antibody (863028)

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Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG ...read more
Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
863028
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Rab13 Antibody (863028) Summary

Immunogen
E. coli-derived recombinant human Rab13
Lys94-Thr191
Accession # P51153
Specificity
Detects human Rab13 in direct ELISA and Western Blot.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RAB13
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB8305 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rab13 Antibody (863028)

  • Cell growth-inhibiting gene 4 protein
  • growth-inhibiting gene 4 protein
  • Rab13
  • RAB13, member RAS oncogene family
  • RAS-associated protein RAB13
  • ras-related protein Rab-13

Background

Ras-related protein Rab13 is a 23 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab13 is detected in endothelial cells and a variety of epithelia, including intestine, lung, kidney and liver.  Rab13 may participate in the assembly and activity of tight junctions, as it colocalizes with the marker ZO-1 in polarized epithelial cells.  In addition, insulin promotes GTP loading of Rab13, potentially regulating GLUT4 translocation in skeletal muscle cells.  Human Rab13 is 203 amino acids (aa) in length, with residues 201-203 removed in its mature form.  Over aa 94-191, human Rab13 shares 89 and 90% identity with mouse and rat Rab13, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab13 Antibody (MAB8305)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol RAB13
Uniprot