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QPRT Antibody (OTI1C10) - Azide and BSA Free

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Western Blot: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY QPRT (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY QPRT.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human lymphoma tissue using anti-QPRT mouse monoclonal antibody.
Flow Cytometry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-QPRT ...read more
Western Blot: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-QPRT monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human endometrium tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human liver tissue using anti-QPRT mouse monoclonal antibody.
Immunohistochemistry: QPRT Antibody (OTI1C10) - Azide and BSA Free [NBP2-73761] - Staining of paraffin-embedded Human prostate tissue using anti-QPRT mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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QPRT Antibody (OTI1C10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human QPRT (NP_055113) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
QPRT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for QPRT Antibody (OTI1C10) - Azide and BSA Free

  • EC 2.4.2.19
  • nicotinate-nucleotide pyrophosphorylase (carboxylating)
  • QAPRTase
  • QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating]
  • Quinolinate phosphoribosyltransferase [decarboxylating]
  • quinolinate phosphoribosyltransferase

Background

QPRT encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QPRT