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Pyruvate Carboxylase Antibody (10S0V1)

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Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726] - Western blot analysis of extracts of various cell lines, using Pyruvate Carboxylase Rabbit mAb (NBP3-16726) at 1:1000 dilution. Secondary antibody: HRP ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
10S0V1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Pyruvate Carboxylase Antibody (10S0V1) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pyruvate Carboxylase (P11498). MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIP
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PC
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Pyruvate Carboxylase Antibody (10S0V1)

  • EC 6.4.1
  • EC 6.4.1.1
  • PCBPyruvic carboxylase
  • Pyruvate Carboxylase
  • pyruvate carboxylase, mitochondrial

Background

PCB encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

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