Recombinant Human PTH Protein Summary
Description |
A single non-glycosylated polypeptide chain containing 78 amino acids corresponding to PTH Source: E. coli Uniprot ID: P01270 Amino Acid Sequence: LMHNLGKHLN SMERVEWLRK KLQDVHNFVA LGAPLAPRDA GSQRPRKKED NVLVESHEKS LGEADKADVN VLTKAKSQ |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
PTH |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of PTH as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
8.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
Alternate Names for Recombinant Human PTH Protein
Background
Parathyroid hormone is secreted by parathyroid cells. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). Most PTH is secreted in its intact, mature 9.5 kDa form, amino acids 1-84; however, it can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. No PTH fragment has been found to be the result of another gene or alternative splicing of the PTH gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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