![Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Western-Blot-NBP1-31435-img0005.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE PRPS2 antibody, antibody diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, Pm, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRPS2. The exact sequence is proprietary. |
| Predicted Species | Mouse (97%), Rat (97%), Porcine (97%), Chimpanzee (98%), Bovine (97%), Chicken (97%), Xenopus (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRPS2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRPS2 Antibody (NBP1-31435)Find related products by research area.
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| Gene Symbol | PRPS2 |
![Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31435-img0003.jpg "Immunocytochemistry/Immunofluorescence: PRPS2 Antibody [NBP1-31435] - Analysis of HaLa, using NBP1-31435 at 1:200 dilution.")
![Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-NBP1-31435-img0007.jpg "Immunohistochemistry: PRPS2 Antibody [NBP1-31435] - Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), and with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, and in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 x 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 x 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 x 1 = 1. Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Immunohistochemistry-Paraffin-NBP1-31435-img0004.jpg "Immunohistochemistry-Paraffin: PRPS2 Antibody [NBP1-31435] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.")
![Knockdown Validated: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRPS2-Antibody-Knockdown-Validated-NBP1-31435-img0006.jpg "Knockdown Validated: PRPS2 Antibody [NBP1-31435] - The effects of PRPS2 knockdown confirmed by qRT-PCR (top) and Western blotting (bottom). Image collected and cropped by CiteAb from the following publication (jcancer.org/v11p1027.htm), licensed under a CC-BY license.")
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202415345324.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 decreased prostate cancer cell growth. (A) The effects of PRPS2 knockdown confirmed by qRT-PCR (top) & Western blotting (bottom). (B) In the CCK‑8 assay, cell viability was decreased in si‑PRPS2 compared with NC. (C) In the plate colony formation assay, colony formation was obviously decreased in si-PRPS2 cells transfected. ***P < 0.001. NC: negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-310202416163792.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - Knockdown of PRPS2 inhibits xenograft tumor growth in vivo. (A) PRPS2 shRNA effectively blocked its protein expression in PC-3 cells. Cells were stably transfected with scramble (sh-Ctrl) or PRPS2 shRNA (sh-PRPS2). PRPS2 protein levels were normalized to alpha -Tubulin. (B) Gross observation of xenograft tumor size in NOD/SCID mice. (C & D) Silencing of PRPS2 inhibited the tumor growth, including tumor volume (P < 0.001) & weight (P = 0.028, n = 6). (E) H&E‑stained paraffin‑embedded sections obtained from xenografts of PC-3 cells. (F) Top: Immunohistochemical analysis of Ki-67 in the xenografts; Bottom: The apoptosis in tumor tissues was evaluated by TUNEL assay (×200). Graphical illustrated the quantification of Ki-67 & TUNEL positive cells percentage. ***P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31435_rabbit-polyclonal-prps2-antibody-3102024161890.jpg "Western Blot: PRPS2 Antibody [NBP1-31435] - PRPS2 expression in prostate cancer tissues & prostate cancer cell lines. (A) Representative immunohistochemistry images of PRPS2 protein expression from the whole TMA with low intensity (b1) in normal prostate tissue (K11, scale bar = 0.2 mm), with low intensity (b4) in prostate cancer tissue (A9, scale bar = 0.2 mm), with intermediate intensity (b3) in prostate cancer tissue (G5, scale bar = 0.2 mm), & with high intensity (b2) in prostate cancer tissue (D2, scale bar = 0.2 mm). The percentage of PRPS2 positive cells in D2 is 90%, in G5 is 70%, & in A9 is 15%. Therefore, the percentage score of the case in D7 is 3 & its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 & its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 & its total protein expression score is 1 × 1 = 1. (B) Western blotting & real-time qPCR analysis of PRPS2 protein & mRNA expression in prostate cancer cell lines & normal prostate epithelial cell lines (RWPE-1). (*P < 0.05; **P < 0.01; ***P < 0.001). PRPS2: Phosphoribosyl pyrophosphate synthetase 2; TMA: tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31956349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")