![Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NB100-55409-img0006.jpg "Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence KELGNSLDKCKNNEN corresponding to internal region according to NP_056444.3. |
| Epitope | KELGNSLDKCKNNEN |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PRPF31 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||||
| Application Notes | WB: Approx. 60 kDa band observed in A431, HeLa, HEK293 and NIH3T3 lysates (calculated MW of 55.4 kDa band according to NP_056444.3). |
||||
| Control |
|
||||
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-55409 | Applications | Species |
|---|---|---|
| Yuan L, Kawada M, Havlioglu N et al. Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells. J Neurosci 2005-01-19 [PMID: 15659613] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRPF31 |
| Entrez | |
| Uniprot |
|
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PRPF31-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55409-img0005.jpg "Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Flow-Cytometry-NB100-55409-img0003.jpg "Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of NIH3T3 nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRPF31-Antibody-Western-Blot-NB100-55409-img0007.jpg "Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of NIH3T3 nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PRPF31-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55409-img0004.jpg "Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
