Histone H4 [ac Lys5] Antibody - BSA Free

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Rat, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 4 of 4.

Publications using NB21-2024	Applications	Species
Franco-García A, Gómez-Murcia V, Fernández-Gómez FJ et al. Morphine-withdrawal aversive memories and their extinction modulate H4K5 acetylation and Brd4 activation in the rat hippocampus and basolateral amygdala Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-06-23 [PMID: 37356373] (ICC/IF, Rat)	ICC/IF	Rat
Rishi JK, Timme K, White HE et al. Obesity partially potentiates dimethylbenz[a]anthracene-exposed ovotoxicity by altering the DNA damage repair response in mice� Biology of reproduction 2023-01-25 [PMID: 36702632] (WB, Mouse) Details: Dilution used in WB 1:500	WB	Mouse
Boltengagen M, Huang A, Boltengagen A et al. A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster Nucleic Acids Res 2015-11-21 [PMID: 26586808] (WB)	WB
Bidne KL. Investigating the ovarian response to endotoxemia. Thesis 1905-07-09 (WB, Porcine)	WB	Porcine

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Pig	11/17/2016	View Summary Application Western Blot Sample Tested Ovary tissue Species Pig Comments Comments Treatment: None. Run on control tissue Controls Positive Control: None Negative Control: Primary only, secondary only, and secondary with IgG all negative Loading Control: Ponceau S stain Total Protein Loaded: 40 ug/well Electrophoresis: Gel Percentage: Gradient, 4-20% Electrophoresis Conditions:      Voltage: 50V then 90V      Time: 5 min then 1 hr Membrane Transfer:      Method (Submersion/Semi-dry): Semi-dry using iBlot2 system      Membrane Type (PVDF/Nitrocellulose): Nitrocellulose      Time: 7 min (iBlot2 Protocol 0)      Voltage: Click here to enter text. Blocking:      Blocking Solution: 5% BSA in .2% PBST      Time: 2.5 hours Primary Antibody:      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST           Incubation Time: overnight           Incubation Temperature: 4°C Washing Conditions:      Wash Solution: .2% PBST      Time and Repetitions: 3X, 10 minutes each Secondary Antibody Manufacturer and Catalog #: Cell Signaling, #7074 Secondary description: goat anti-rabbit      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST      Incubation Time: 1 hour      Incubation Temperature: room temperature Detection Method: Detection: SignalFire ECL reagent (Cell Signaling) Procedure: ECL on blot 3 min Development Time: 5 min Molecular weight of band(s): 13 kDa
	5 reviewed by: Verified Customer	WB	Human	10/04/2015	View Summary Application Western Blot Sample Tested Human cancer cell Species Human

Array NB21-2024

• Histone H4 Antibodies
• Histone H4 Lysate
• Histone H4 Proteins