Home » Prox1 » Prox1 Lysates » Prox1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Prox1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: PROX1 Overexpression Lysate (Adult Normal) [NBL1-14814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PROX1.
Western Blot: PROX1 Overexpression Lysate (Adult Normal) [NBL1-14814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PROX1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Prox1 Overexpression Lysate Summary

Description

Prox1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201140

Accession#: NM_002763

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
PROX1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Prox1 Overexpression Lysate

  • Homeobox prospero-like protein PROX1
  • prospero homeobox 1
  • prospero homeobox protein 1
  • prospero-related homeobox 1
  • Prox1
  • PROX-1

Background

PROX1 may play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. PROX1 antibodies are also used as a highly specific marker of lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

VEGFR3/Flt-4
Antibody [Unconjugate
AF743
Flow Cytometry VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
(3 Reviews)
(139 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
LYVE-1 Antibody
[Unconjugated]
AF2125
Immunohistochemistry LYVE-1 Antibody [Unconjugated]
Western Blot LYVE-1 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence LYVE-1 Antibody [Unconjugated]
(1 Review)
(139 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
VEGF-C [HRP]
DVEC00
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
(53 Publications)
Species: Hu
Applications: ELISA
Pax6 Antibody
[Unconjugated]
AF8150
Simple Western Pax6 Antibody [Unconjugated]
Immunocytochemistry Pax6 Antibody [Unconjugated]
Western Blot Pax6 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: ICC, ICFlow, Simple Western, WB
Podoplanin
Antibody [Unconjugate
AF3244
Western Blot Podoplanin Antibody [Unconjugated]
Immunohistochemistry Podoplanin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Podoplanin Antibody [Unconjugated]
(1 Review)
(74 Publications)
Species: Mu
Applications: IHC, Simple Western, WB
p57 Kip2 Antibody
NBP1-89917
Immunohistochemistry-Paraffin p57 Kip2 Antibody
Immunohistochemistry-Paraffin p57 Kip2 Antibody
Immunohistochemistry-Paraffin p57 Kip2 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(681 Publications)
Species: Hu
Applications: ELISA
CCL16/HCC-4/LEC
802-HC/CF
CCL16/HCC-4/LEC
(6 Publications)
Species: Hu
Applications: BA, BA
LRH-1/NR5A2
Antibody
NBP2-27196
Immunohistochemistry-Paraffin LRH-1/NR5A2 Antibody
Western Blot LRH-1/NR5A2 Antibody
(1 Publication)
Species: Bv, Ca, Ch, ChHa, Eq, Gp, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, RM, Ze
Applications: IHC, IHC-P, KD, WB
c-Maf Antibody -
BSA Free
NBP2-24551
Immunohistochemistry-Paraffin c-Maf Antibody - BSA Free
Western Blot c-Maf Antibody - BSA Free
Western Blot c-Maf Antibody - BSA Free
(3 Reviews)
(2 Publications)
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SOX2 Antibody
[Unconjugated]
AF2018
Western Blot SOX2 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]
Simple Western SOX2 Antibody [Unconjugated]
(6 Reviews)
(368 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ICC, IHC, Simple Western, WB
SIX3 Antibody (1A2)
H00006496-M04
Western Blot SIX3 Antibody (1A2)
Western Blot SIX3 Antibody (1A2)
Western Blot SIX3 Antibody (1A2)
Species: Hu
Applications: ELISA, WB
Coronin-1a Antibody
NB110-58867
Western Blot Coronin-1a Antibody
Immunocytochemistry/ Immunofluorescence Coronin-1a Antibody
(4 Publications)
Species: Bv, Hu, Mu, Po, Rt
Applications: ICC/IF, WB
Hemoglobin alpha
Antibody - BSA Free
NB110-41083
Hemoglobin alpha Antibody - BSA Free
(12 Publications)
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
CD31/PECAM-1
Antibody [Unconjugate
AF3628
Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]
Flow Cytometry CD31/PECAM-1 Antibody [Unconjugated]
Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]
(6 Reviews)
(494 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
FOXE3 Antibody
NBP3-10932
Immunohistochemistry FOXE3 Antibody
Species: Hu
Applications: IHC, WB
VEGF-D [HRP]
DVED00
N/A VEGF-D [HRP]
N/A VEGF-D [HRP]
N/A VEGF-D [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
ASCL1/Mash1
Antibody [Unconjugate
AF2567
Immunohistochemistry ASCL1/Mash1 Antibody [Unconjugated]
(2 Publications)
Species: Mu
Applications: IHC, WB
Prox1
Overexpression Lysate
NBL1-14814
Prox1 Overexpression Lysate
Species: Hu
Applications: WB

Publications for Prox1 Lysate (NBL1-14814) (0)

There are no publications for Prox1 Lysate (NBL1-14814).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Prox1 Lysate (NBL1-14814) (0)

There are no reviews for Prox1 Lysate (NBL1-14814). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Prox1 Lysate (NBL1-14814) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Prox1 Products

Research Areas for Prox1 Lysate (NBL1-14814)

Find related products by research area.

Blogs on Prox1.

Check out the latest blog posts on Prox1.

  Read full blog post.
Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Prox1 Antibody [Unconjugated]
AF2727
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
CD31/PECAM-1 Antibody [Unconjugated]
AF3628

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Prox1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol PROX1